PurposeLocalization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||55100||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneVCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL)
- Promoter CMV
/ Fusion Proteins
- mCherry (N terminal on backbone)
- miniSOG (N terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Excitation = 448 / 473; Emission = 500 / 528
Please note that miniSOG is upstream of mCherry and Vinculin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mCherry-miniSOG-Vinculin-23 was a gift from Michael Davidson (Addgene plasmid # 55100)
For your References section:A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms. Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, Ellisman MH, Tsien RY. PLoS Biol. 2011 Apr;9(4):e1001041. doi: 10.1371/journal.pbio.1001041. Epub 2011 Apr 5. 10.1371/journal.pbio.1001041 PubMed 21483721