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Kinetics and epigenetics of retroviral silencing in mouse embryonic stem cells defined by deletion of the D4Z4 element.
Rival-Gervier S, Lo MY, Khattak S, Pasceri P, Lorincz MC, Ellis J
Mol Ther. 2013 Aug;21(8):1536-50. doi: 10.1038/mt.2013.131. Epub 2013 Jun 11. PubMed Article

Plasmids from Article

ID Plasmid Purpose
58254HSC1-GiPThis self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette
58540HSC1-HS4-GiPDerived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR
58541HSC1-HS4-D4Z4c-GiPDerived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter
58542HSC1-D4Z4-GiPDerived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter

Antibodies from Article