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Versatile Recombinant SUMOylation System for the Production of SUMO-Modified Protein.
Weber AR, Schuermann D, Schar P
PLoS One. 2014 Jul 9;9(7):e102157. doi: 10.1371/journal.pone.0102157. eCollection 2014.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
52258pSUMO1Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2
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52259pSUMO2Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2
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52260pSUMO3Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2
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52261pSA1Bacterial expression of human 6His-tagged SUMO1 and SUMO-E1
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52262pSA2Bacterial expression of human 6His-tagged SUMO2 and SUMO-E1
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52263pSA3Bacterial expression of human 6His-tagged SUMO3 and SUMO-E1
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52264pSC-PreE2Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker
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52265pSC-IntE2Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linker
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52266pTG-mTDGBacterial expression of mouse TDG with C-terminal GST-tag
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52267pTG-mTDG-K341RBacterial expression of mouse TDG-K341R with C-terminal GST-tag
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52268pSC-mTDG-IntE2Bacterial expression of mouse TDG C-terminally fused to GyrA intein, SUMO-E2 and GST
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52269pSC-mTDG-K341R-IntE2Bacterial expression of mouse TDG-K341R C-terminally fused to GyrA intein, SUMO-E2 and GST
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52270pSC-hTDG-IntE2Bacterial expression of human TDG C-terminally fused to GyrA intein, SUMO-E2 and GST
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52271pSC-hTDG-K330A-IntE2Bacterial expression of human TDG-K330A C-terminally fused to GyrA intein, SUMO-E2 and GST
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52272pSC-hTDG-PreE2Bacterial expression of human TDG C-terminally fused to SUMO-E2 and GST
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52273pSC-hTDG-K330A-PreE2Bacterial expression of human TDG-K330A C-terminally fused to SUMO-E2 and GST
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52274pGEX-hXRCC1Bacterial expression of human XRCC1 with N-terminal GST-tag
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52275pGEX-hXRCC1-K176RBacterial expression of human XRCC1-K176R with N-terminal GST-tag
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52276pSC-hXRCC1-IntE2Bacterial expression of human XRCC1 C-terminally fused to GyrA intein, SUMO-E2 and GST
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52277pSC-hXRCC1-K176R-IntE2Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein, SUMO-E2 and GST
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52278pSC-hXRCC1-PreE2Bacterial expression of human XRCC1 C-terminally fused to SUMO-E2 and GST
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52279pSC-hXRCC1-K176R-PreE2Bacterial expression of human XRCC1-K176R C-terminally fused to SUMO-E2 and GST
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52280pTXGBacterial expression of TARGET proteins C-terminally fused to GyrA intein and GST
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52281pTXG-hTDGBacterial expression of human TDG C-terminally fused to GyrA intein and GST
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52282pTXG-hTDG-K330ABacterial expression of human TDG-K330A C-terminally fused to GyrA intein and GST
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52283pTXG-hXRCC1Bacterial expression of human XRCC1 C-terminally fused to GyrA intein and GST
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52284pTXG-hXRCC1-K176RBacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein and GST
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Recombinant Antibodies from Article