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Regulation of microtubule motors by tubulin isotypes and post-translational modifications.
Sirajuddin M, Rice LM, Vale RD
Nat Cell Biol. 2014 Apr;16(4):335-44. doi: 10.1038/ncb2920. Epub 2014 Mar 16.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
60385pRS426-TUB1Expression of yeast alpha tubulin (TUB1) using a GAL promoter
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60386pRS424-TUB2-Cterminal6xHisExpression of yeast beta tubulin (TUB2)-Cterminal 6xHis using a GAL promoter
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60387pRS424-TUB1-Cterminal6xHisExpression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a GAL promoter
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60388pRS424-TUB2Expression of yeast beta tubulin (TUB2) using a GAL promoter
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60390pRS426-TUB1-internal6xHisExpression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter
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60391pRS426-TUB1-internal6xHis (1-440)Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter
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60392pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tailExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoter
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60393pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta YExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)
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60394pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446CExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptide
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60395pRS424-TUB2 (1-428)Expression of yeast beta tubulin (TUB2) - without Cterminal tail using GAL promoter
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60396pRS424-TUB2 - human TUBB2a Cterminal tailExpression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoter
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60397pRS424-TUB2 - human TUBB3 Cterminal tailExpression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoter
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60398pRS424-TUB2 - human TUBB2a Cterminal tail C127S, E435CExpression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) C127S, E435C for crosslinking polyglutamate peptide
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60399pRS424-TUB2 - human TUBB7 Cterminal tailExpression of chimeric yeast beta tubulin (TUB2) - human beta7 tubulin Cterminal tail (TUBB7) using a GAL promoter
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60400pRS424-TUB2 - human TUBB1 Cterminal tailExpression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoter
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60401pRS424-TUB2 - human TUBB8 Cterminal tailExpression of chimeric yeast beta tubulin (TUB2) - human beta8 tubulin Cterminal tail (TUBB8) using a GAL promoter
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60402pRS424-TUB2 - human TUBB4a Cterminal tailExpression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) using a GAL promoter
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60403pRS424-TUB2 - human TUBB6 Cterminal tailExpression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter
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60404pRS424-TUB2 - human TUBB3 Cterminal tail delta KExpression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPK
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60405pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435CExpression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptide
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60406pHR KIF17 (1-738)Expression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738
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Recombinant Antibodies from Article