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CRISPR/Cas9 allows efficient and complete knock-in of a destabilization domain-tagged essential protein in a human cell line, allowing rapid knockdown of protein function.
Park A, Won ST, Pentecost M, Bartkowski W, Lee B
PLoS One. 2014 Apr 17;9(4):e95101. doi: 10.1371/journal.pone.0095101. eCollection 2014.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
60747PuroR-P2A-DD-TCOF1-donorDonor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1
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60748BlastR-P2A-DD-TCOF1-donorDonor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1
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