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Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells.
Niopek D, Benzinger D, Roensch J, Draebing T, Wehler P, Eils R, Di Ventura B
Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
61342pDB22Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)
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61343pDN34mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)
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61344pDN41mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)
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61345pDN42mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)
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61346pDN43mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES)
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61347pDN77mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkB╬▒ NES)
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61349pDN80LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.
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