A split-Cas9 architecture for inducible genome editing and transcription modulation.
Zetsche B, Volz SE, Zhang F
Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. PubMed Article
Zetsche B, Volz SE, Zhang F
Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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62883 | PX851 | N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4). |
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62884 | PX852 | C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4). |
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62885 | PX853 | N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5). |
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62886 | PX854 | C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5). |
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62887 | PX855 | N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES) |
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62888 | PX856 | C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64) |
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62889 | pLSC-5 | Lenti vector for expression of inducible split Cas9 |
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