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A novel assay reveals preferential binding between Rabs, kinesins, and specific endosomal subpopulations.
Bentley M, Decker H, Luisi J, Banker G
J Cell Biol. 2015 Feb 2;208(3):273-281. Epub 2015 Jan 26. PubMed Article

Plasmids from Article

ID Plasmid Purpose
63569pBa-eGFP-flag-BicD2 594-FKBPBicaudal D protein aa1-594 (excluding start Met) tagged with N-terminal eGFP and flag and a C-terminal FKBP
64205pBa-tdTomato-flag-BicD2 594-FKBPBicaudal D protein aa1-594 (excluding start Met) with N-terminal tandem dimer Tomato and flag tags and C-terminal FKBP
64206pBa-flag-BicD2 594-FKBPBicaudal D protein aa1-594 (excluding start Met) with N-terminal flag tag and C-terminal FKBP
64209pBa-FRB-3myc-Rab5aRab5a with N-terminal FRB and triple myc tags
64210pBa-FRB-3myc-Rab7Rab7 with N-terminal FRB and triple myc tags
64211pBa-KIF5C 559-tdTomato-FKBPKIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags
64286pBa-FRB-3myc-KIF1A tail 391-1698KIF1A aa 391-1698 with N-terminal FRB and triple myc tags
64287pBa-FRB-3myc-KIF1B beta tail 387-1770KIF1B beta aa 387-1770 with N-terminal FRB and triple myc tags
64288pBa-FRB-3myc-KIF13A tail 361-1749KIF13A aa 361-1749 with N-terminal FRB and triple myc tags
64289pBa-FRB-3myc-KIF13B tail 442-1826KIF13A aa 442-1826 with N-terminal FRB and triple myc tags

Antibodies from Article