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Christopher A Walsh Lab Plasmids


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Title Authors Publication
Evolutionarily dynamic alternative splicing of GPR56 regulates regional cerebral cortical patterning. Bae BI, Tietjen I, Atabay KD, Evrony GD, Johnson MB, Asare E, Wang PP, Murayama AY, Im K, Lisgo SN, Overman L, Sestan N, Chang BS, Barkovich AJ, Grant PE, Topcu M, Politsky J, Okano H, Piao X, Walsh CA Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392.
Walsh Lab unpublished plasmids (unpublished)
Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
pCMV-HA (New MCS) Mammalian Expression Walsh Lab unpublished plasmids (unpublished) pCMV-HA
  • Tag / Fusion Protein
    • HA (N terminal on backbone)
  • Add to Cart
    pCAG-DN-RFX Dominant negative RFX (Mus musculus) Mammalian Expression Evolutionarily dynamic alternative splicing of GPR56 regulates regional cerebral cortical patterning. Bae et al Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392. Dominant-negatively inhibits RFX (regulatory factor X) transcription factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and C-terminal myc tag. pCAGEN Add to Cart
    pCAG-hGPR56-IRES-GFP Human GPR56 coding sequence (Homo sapiens) Mammalian Expression Evolutionarily dynamic alternative splicing of GPR56 regulates regional cerebral cortical patterning. Bae et al Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392. Expresses human GPR56 and GFP in mammalian cells. pCAGIG GPR56 UNQ540/PRO1083, BFPP, TM7LN4, TM7XN1 Add to Cart
    pGL3E-hGPR56 e1m promoter normal Human GPR56 e1m promoter (Homo sapiens) Luciferase Evolutionarily dynamic alternative splicing of GPR56 regulates regional cerebral cortical patterning. Bae et al Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392. Reporter for normal human GPR56 exon 1m promoter activity. Human GPR56 e1m promoter (chr16:56,230,630-56,230,943 (hg18)) was inserted into pGL3E. pGL3E Add to Cart
    pGL3E-hGPR56 e1m promoter perisylvian polymicrogyria Human GPR56 e1m promoter (Homo sapiens) Luciferase Evolutionarily dynamic alternative splicing of GPR56 regulates regional cerebral cortical patterning. Bae et al Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392. Reporter for mutated human GPR56 exon 1m promoter activity. It contains a 15-bp deletion in a conserved noncoding element. The mutated human GPR56 e1m promoter was inserted into pGL3E. pGL3E Add to Cart