Fire Lab C. elegans Vector Kit Icon

Fire Lab C. elegans Vector Kit
(Kit # 1000000001 )

Depositing Labs:   Andrew Fire

Dr. Andrew Fire's lab has deposited a set of vectors for C. elegans research. This kit contains 288 vectors and includes both a general description and the full predicted DNA sequence for each vector.

This kit consists of 3 plates (288 plasmids), and will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs. Addgene does not sell worm strains.

$375 USD + shipping
Available to Academic and Nonprofits Only

Description

Dr. Andrew Fire's lab has deposited a set of vectors for C. elegans research. This kit contains 288 vectors and includes both a general description and the full predicted DNA sequence for each vector. Among these vectors are:

  • lacZ and/or GFP fusion vectors with higher expression efficiency than many earlier C. elegans vectors. These include vectors with coding region for blue-shifted GFP, cyan-shifted GFP, yellow-shifted GFP, and other GFP protein variants.

  • Vectors for tagging C. elegans exons and introns with GFP.

  • Control plasmids for RNA-mediated interference.

  • Hairpin sequences to test promoters for ability to trigger RNAi in-vivo.

Kit Documentation

Icon Fire Lab C. elegans Vector Kit Info Addgene (238.8 KB)


The vectors in this kit are divided into 3 plates (96 samples each). The sample plate location information and documentaion are as follows:

Additional documents from the Fire lab:

More Downloadable Documentation

In these kit documentations, plasmids may be referred by their mini-prep number (prefix pPD), or ligation number (prefix L). Please consult the Excel file below for translation between these naming schemes. In the Addgene library, you may search for a plasmid using either number.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The Fire Lab C. elegans Vector Kit was a gift from Andrew Fire (Addgene kit # 1000000001)"

Resistance Color Key:

 
  Ampicillin  
 
  Kanamycin  

Inventory

Well Plasmid Resistance
1 / A / 1 pPD87_18
  Ampicillin
1 / A / 2 pPD90_23
  Ampicillin
1 / A / 3 pPD95_67
  Ampicillin
1 / A / 4 pPD80_08
  Ampicillin
1 / A / 5 pPD88_22
  Ampicillin
1 / A / 6 pPD90_28
  Ampicillin
1 / A / 7 pPD95_70
  Ampicillin
1 / A / 8 pPD95_93
  Ampicillin
1 / A / 9 pPD87_50
  Ampicillin
1 / A / 10 pPD90_31
  Ampicillin
1 / A / 11 pPD95_69
  Ampicillin
1 / A / 12 pPD96_02
  Ampicillin
1 / B / 1 pPD88_27
  Ampicillin
1 / B / 2 pPD89_03
  Ampicillin
1 / B / 3 pPD95_73
  Ampicillin
1 / B / 4 pPD96_04
  Ampicillin
1 / B / 5 pPD91_14
  Ampicillin
1 / B / 6 pPD89_09
  Ampicillin
1 / B / 7 pPD95_79
  Ampicillin
1 / B / 8 pOK11_34
  Ampicillin
1 / B / 9 pPD95_15
  Ampicillin
1 / B / 10 pPD89_20
  Ampicillin
1 / B / 11 pPD95_77
  Ampicillin
1 / B / 12 pPD95_62
  Ampicillin
1 / C / 1 pPD88_75
  Ampicillin
1 / C / 2 pPD89_17
  Ampicillin
1 / C / 3 pPD95_75
  Ampicillin
1 / C / 4 pPD96_62
  Ampicillin
1 / C / 5 pPD96_12
  Ampicillin
1 / C / 6 pPD95_03
  Ampicillin
1 / C / 7 pPD95_81
  Ampicillin
1 / C / 8 pPD69_39
  Ampicillin
1 / C / 9 pPD93_51
  Ampicillin
1 / C / 10 pPD95_07
  Ampicillin
1 / C / 11 pPD95_85
  Ampicillin
1 / C / 12 pPD95_16
  Ampicillin
1 / D / 1 pPD93_48
  Ampicillin
1 / D / 2 pPD95_57
  Ampicillin
1 / D / 3 pPD96_32
  Ampicillin
1 / D / 4 pPD95_21
  Ampicillin
1 / D / 5 pPD93_65
  Ampicillin
1 / D / 6 pPD95_10
  Ampicillin
1 / D / 7 pPD79_44
  Ampicillin
1 / D / 8 pPD95_25
  Ampicillin
1 / D / 9 pPD94_81
  Ampicillin
1 / D / 10 pPD95_11
  Ampicillin
1 / D / 11 pPD95_02
  Ampicillin
1 / D / 12 pPD95_27
  Ampicillin
1 / E / 1 pPD47_52
  Kanamycin
1 / E / 2 pPD10_46
  Ampicillin
1 / E / 3 pPD50_21
  Ampicillin
1 / E / 4 pICT19h
  Ampicillin
1 / E / 5 pPD57_56
  Ampicillin
1 / E / 6 pPD26_77
  Ampicillin
1 / E / 7 pPD20_97
  Ampicillin
1 / E / 8 pAST_19a
  Ampicillin
1 / E / 9 pPD8_02
  Ampicillin
1 / E / 10 pPD34_110
  Ampicillin
1 / E / 11 pPD50_14
  Ampicillin
1 / E / 12 pAST_18b
  Ampicillin
1 / F / 1 pPD8_33
  Ampicillin
1 / F / 2 pPD81_34
  Ampicillin
1 / F / 3 pPD49_78
  Ampicillin
1 / F / 4 pPD16_43
  Ampicillin
1 / F / 5 pPD61_125
  Ampicillin
1 / F / 6 pPD49_83
  Ampicillin
1 / F / 7 pPD16_51
  Ampicillin
1 / F / 8 pPD93_97
  Ampicillin
1 / F / 9 pPD30_69
  Ampicillin
1 / F / 10 pPD95_18
  Ampicillin
1 / F / 11 pPD52_37
  Ampicillin
1 / F / 12 pPD18_32
  Ampicillin
1 / G / 1 pPD95_86
  Ampicillin
1 / G / 2 pPD54_01
  Ampicillin
1 / G / 3 pPD21_28
  Ampicillin
1 / G / 4 pPD5_41
  Ampicillin
1 / G / 5 pPD52_102
  Ampicillin
1 / G / 6 pPD22_11
  Ampicillin
1 / G / 7 pPD30_35
  Ampicillin
1 / G / 8 pPD22_04
  Ampicillin
1 / G / 9 pICT19r
  Ampicillin
1 / G / 10 pPD18_49
  Ampicillin
1 / G / 11 pPD30_38
  Ampicillin
1 / G / 12 pDD16_01
  Ampicillin
1 / H / 1 pPD152.79
  Ampicillin
1 / H / 2 pPD157.60
  Ampicillin
1 / H / 3 pPD160.60
  Ampicillin
1 / H / 4 pPD158.87
  Ampicillin
1 / H / 5 pPD157.63
  Ampicillin
1 / H / 6 pPD117.20
  Ampicillin
1 / H / 7 pPD157.99
  Ampicillin
1 / H / 8 pPD138.11
  Ampicillin
1 / H / 9 pPD132.49
  Ampicillin
1 / H / 10 pPD172.01
  Ampicillin
1 / H / 11 pPD176.36
  Ampicillin
1 / H / 12 pPD176.86
  Ampicillin
2 / A / 1 L2603
  Ampicillin
2 / A / 2 L3567
  Ampicillin
2 / A / 3 L2604
  Ampicillin
2 / A / 4 L3568
  Ampicillin
2 / A / 5 L2605
  Ampicillin
2 / A / 6 L3569
  Ampicillin
2 / A / 7 L2630
  Ampicillin
2 / A / 8 L3570
  Ampicillin
2 / A / 9 L2678
  Ampicillin
2 / A / 10 L3571
  Ampicillin
2 / A / 11 L2680
  Ampicillin
2 / A / 12 L3572
  Ampicillin
2 / B / 1 L3573
  Ampicillin
2 / B / 2 L2681
  Ampicillin
2 / B / 3 L3574
  Ampicillin
2 / B / 4 L2682
  Ampicillin
2 / B / 5 L3602
  Ampicillin
2 / B / 6 L2684
  Ampicillin
2 / B / 7 L3603
  Ampicillin
2 / B / 8 L2685
  Ampicillin
2 / B / 9 L3604
  Ampicillin
2 / B / 10 L2822
  Ampicillin
2 / B / 11 L3605
  Ampicillin
2 / B / 12 L2865
  Ampicillin
2 / C / 1 L2908
  Ampicillin
2 / C / 2 L3606
  Ampicillin
2 / C / 3 L2911
  Ampicillin
2 / C / 4 L3607
  Ampicillin
2 / C / 5 L2944
  Ampicillin
2 / C / 6 L3608
  Ampicillin
2 / C / 7 L2946
  Ampicillin
2 / C / 8 L3609
  Ampicillin
2 / C / 9 L2947
  Ampicillin
2 / C / 10 L3610
  Ampicillin
2 / C / 11 L2963
  Ampicillin
2 / C / 12 L3611
  Ampicillin
2 / D / 1 L3612
  Ampicillin
2 / D / 2 L3110
  Ampicillin
2 / D / 3 L3613
  Ampicillin
2 / D / 4 L3111
  Ampicillin
2 / D / 5 L3615
  Ampicillin
2 / D / 6 L3135
  Ampicillin
2 / D / 7 L3616
  Ampicillin
2 / D / 8 L3136
  Ampicillin
2 / D / 9 L3617
  Ampicillin
2 / D / 10 L3137
  Ampicillin
2 / D / 11 L3618
  Ampicillin
2 / D / 12 L3465
  Ampicillin
2 / E / 1 L3467
  Ampicillin
2 / E / 2 L3619
  Ampicillin
2 / E / 3 L3469
  Ampicillin
2 / E / 4 L3620
  Ampicillin
2 / E / 5 L3471
  Ampicillin
2 / E / 6 L3621
  Ampicillin
2 / E / 7 L3502
  Ampicillin
2 / E / 8 L3691
  Ampicillin
2 / E / 9 L3503
  Ampicillin
2 / E / 10 L3774
  Ampicillin
2 / E / 11 L3505
  Ampicillin
2 / E / 12 L3781
  Ampicillin
2 / F / 1 L3784
  Ampicillin
2 / F / 2 L3506
  Ampicillin
2 / F / 3 L3785
  Ampicillin
2 / F / 4 L3513
  Ampicillin
2 / F / 5 L3786
  Ampicillin
2 / F / 6 L3514
  Ampicillin
2 / F / 7 L3787
  Ampicillin
2 / F / 8 L3515
  Ampicillin
2 / F / 9 L3788
  Ampicillin
2 / F / 10 L3516
  Ampicillin
2 / F / 11 L3790
  Ampicillin
2 / F / 12 L3517
  Ampicillin
2 / G / 1 L3522
  Ampicillin
2 / G / 2 L3812
  Ampicillin
2 / G / 3 L3527
  Ampicillin
2 / G / 4 L3799
  Ampicillin
2 / G / 5 L3557
  Ampicillin
2 / G / 6 L3827
  Ampicillin
2 / G / 7 L3558
  Ampicillin
2 / G / 8 L3828
  Ampicillin
2 / G / 9 L3559
  Ampicillin
2 / G / 10 L3829
  Ampicillin
2 / G / 11 L3560
  Ampicillin
2 / G / 12 L3863
  Ampicillin
2 / H / 1 L3809
  Ampicillin
2 / H / 2 L3561
  Ampicillin
2 / H / 3 L3670
  Ampicillin
2 / H / 4 L3562
  Ampicillin
2 / H / 5 L3808
  Ampicillin
2 / H / 6 L3563
  Ampicillin
2 / H / 7 L3811
  Ampicillin
2 / H / 8 L3564
  Ampicillin
2 / H / 9 L3720
  Ampicillin
2 / H / 10 L3565
  Ampicillin
2 / H / 11 BK48
  Ampicillin
2 / H / 12 L3566
  Ampicillin
3 / A / 1 L4816
  Ampicillin
3 / A / 2 L4053
  Ampicillin
3 / A / 3 L4663
  Ampicillin
3 / A / 4 L4051
  Ampicillin
3 / A / 5 L4686
  Ampicillin
3 / A / 6 L4040
  Ampicillin
3 / A / 7 L4665
  Ampicillin
3 / A / 8 L4037
  Ampicillin
3 / A / 9 L4664
  Ampicillin
3 / A / 10 L4042
  Ampicillin
3 / A / 11 L4682
  Ampicillin
3 / A / 12 L4043
  Ampicillin
3 / B / 1 L4038
  Ampicillin
3 / B / 2 L4660
  Ampicillin
3 / B / 3 L4453
  Ampicillin
3 / B / 4 L4780
  Ampicillin
3 / B / 5 L4455
  Ampicillin
3 / B / 6 L4752
  Ampicillin
3 / B / 7 L4759
  Ampicillin
3 / B / 8 L4817
  Ampicillin
3 / B / 9 L4796
  Ampicillin
3 / B / 10 L4643
  Ampicillin
3 / B / 11 L4809
  Ampicillin
3 / B / 12 L4687
  Ampicillin
3 / C / 1 L4667
  Ampicillin
3 / C / 2 L4057
  Ampicillin
3 / C / 3 L4666
  Ampicillin
3 / C / 4 L4058
  Ampicillin
3 / C / 5 L4683
  Ampicillin
3 / C / 6 L4035
  Ampicillin
3 / C / 7 L4671
  Ampicillin
3 / C / 8 L453
  Ampicillin
3 / C / 9 L4781
  Ampicillin
3 / C / 10 L4129
  Ampicillin
3 / C / 11 L4753
  Ampicillin
3 / C / 12 L4119
  Ampicillin
3 / D / 1 L4138
  Ampicillin
3 / D / 2 L4640
  Ampicillin
3 / D / 3 L4819
  Ampicillin
3 / D / 4 L4061
  Ampicillin
3 / D / 5 L4440
  Ampicillin
3 / D / 6 L4062
  Ampicillin
3 / D / 7 pLT61
  Ampicillin
3 / D / 8 L4063
  Ampicillin
3 / D / 9 pLT63
  Ampicillin
3 / D / 10 L4064
  Ampicillin
3 / D / 11 L4417
  Ampicillin
3 / D / 12 L4065
  Ampicillin
3 / E / 1 L4066
  Ampicillin
3 / E / 2 L4400
  Ampicillin
3 / E / 3 L4095
  Ampicillin
3 / E / 4 L4271
  Ampicillin
3 / E / 5 L4096
  Ampicillin
3 / E / 6 L4404
  Ampicillin
3 / E / 7 L4458
  Ampicillin
3 / E / 8 pPD191.45
  Ampicillin
3 / E / 9 L4506
  Ampicillin
3 / E / 10 L4431
  Ampicillin
3 / E / 11 L4507
  Ampicillin
3 / E / 12 L4842
  Ampicillin
3 / F / 1 L4437
  Ampicillin
3 / F / 2 L4632
  Ampicillin
3 / F / 3 L4438
  Ampicillin
3 / F / 4 L4054
  Ampicillin
3 / F / 5 pBK125
  Ampicillin
3 / F / 6 L4052
  Ampicillin
3 / F / 7 pPD187.49
  Ampicillin
3 / F / 8 L4019
  Ampicillin
3 / F / 9 L754
  Ampicillin
3 / F / 10 L4047
  Ampicillin
3 / F / 11 L2528
  Ampicillin
3 / F / 12 L4018
  Ampicillin
3 / G / 1 L4046
  Ampicillin
3 / G / 2 L2534
  Ampicillin
3 / G / 3 L4020
  Ampicillin
3 / G / 4 L2531
  Ampicillin
3 / G / 5 L4048
  Ampicillin
3 / G / 6 L3929
  Ampicillin
3 / G / 7 L4041
  Ampicillin
3 / G / 8 L3928
  Ampicillin
3 / G / 9 L4026
  Ampicillin
3 / G / 10 L3930
  Ampicillin
3 / G / 11 L4049
  Ampicillin
3 / G / 12 L3920
  Ampicillin
3 / H / 1 L3927
  Ampicillin
3 / H / 2 pJKL464
  Ampicillin
3 / H / 3 pPD177.53
  Ampicillin
3 / H / 4 pPD177.62
  Ampicillin
3 / H / 5 pPD177.66
  Ampicillin
3 / H / 6 pPD177.71
  Ampicillin
3 / H / 7 pPD187.24
  Ampicillin
3 / H / 8 pPD159.88
  Ampicillin
3 / H / 9 pPD107.09
  Ampicillin
3 / H / 10 pPD158.114
  Ampicillin
3 / H / 11 pPD158.117
  Ampicillin
3 / H / 12 pPD159.62
  Ampicillin
Data from: CALCULATED @ 2014-11-24 15:12:23.900973