Fire Lab C. elegans Vector Kit
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Dr. Andrew Fire's lab has deposited a set of vectors for C. elegans research. These vectors are available as a kit, or can be ordered individually. Addgene does not distribute worm strains.
This kit contains 288 vectors and includes both a general description and the full predicted DNA sequence for each vector. Among these vectors are:
- lacZ and/or GFP fusion vectors with higher expression efficiency than many earlier C. elegans vectors. These include vectors with coding region for blue-shifted GFP, cyan-shifted GFP, yellow-shifted GFP, and other GFP protein variants.
- Vectors for tagging C. elegans exons and introns with GFP.
- Control plasmids for RNA-mediated interference.
- Hairpin sequences to test promoters for ability to trigger RNAi in-vivo.
- And more…
Documentation
The vectors are divided into 3 plates (96 samples each):
- 1995 plate (blue colored caps) Plate Map, Documentation
- 1997 plate (yellow colored caps) Plate Map, Documentation
- 1999 plate (green colored caps) Plate Map, Documentation
- 2005 vectors: H1 through H12 of the 1995 plate, H3 through H12 of the 1999 plate, and E8 and F7 of the 1999 plate are 24 new vectors made available in 2005. These vectors were not available in the 1995 and 1999 vector sets previously distributed by the Fire lab. Documentation.
Other Documents:
- PDF file of all plasmid information and maps (8 MB, no sequences).
- Excel file summarizing plasmid features, sequences, and plate locations (2.6 MB).
- Zip file containing a folder that includes plasmid features and sequences for each plasmid as individual files (774 KB).
- Text file containing all sequences in GenBank format (3 MB).
- Plasmid information and sequences are also packaged into a Hypercard application called DNA Janitor (472 KB zip/StuffIt). DNA Janitor, written by Dr. Fire, provides interfaces for browsing and searching plasmid information. This application requires a HyperCard viewer (Download from Apple) to run on either MacOS X Classic Mode, or MacOS 9 or earlier.
In the documentations, plasmids may be referred by their mini-prep number (prefix pPD), or ligation number (prefix L). Please consult the above Excel file for translation between these naming schemes. On this website, you may search for a plasmid using either number.
Distribution and Handling
You may order individual plasmids from the kit as well as the entire kit. Addgene does not sell worm strains.
Individual Plasmids Addgene charges $40 for each vector, plus shipping and handling. This is a reduced price courtesy of Dr. Fire. Browse Plasmids Within the Kit
Addgene ships individual plasmids as bacterial stabs at room temperature.
The Entire Kit (catalog#1000000001): Addgene charges $375 for the entire kit, plus shipping and handling.
Addgene ships the kit as 288 bacterial glycerol stocks, in 96-well format, on dry ice. Samples should be frozen at -80oC immediately upon arrival.
Note that Addgene uses UPS for shipments and can ship dry ice to most countries, excluding China. Scientists from China interested in obtaining this kit should contact help@addgene.org.
The following instructions are included with the kit:
Request Plasmids
To obtain a plasmid, Login or Register a new account. Find your plasmid of interest, then click the Add to Cart button. When you are finished adding plasmids to your cart, complete the checkout process online. Click here for Addgene’s pricing and distribution policy.
Photo Credit: James McCarter, Genome Sequencing Center