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L4440
(Plasmid #1654)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1654 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Cloning Grade DNA 1654-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $105

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Unknown
  • Backbone size (bp) 2790
  • Vector type
    Worm Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • T7p, T7p, lacZN, OriF1>>, OriF1<<

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD129.36, Ligation number L4440. "Author's Map" courtesy of Dr. Guy Caldwell.

Information for Cloning Grade DNA (Catalog # 1654-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $105 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L4440 was a gift from Andrew Fire (Addgene plasmid # 1654 ; http://n2t.net/addgene:1654 ; RRID:Addgene_1654)