Addgene - lis-1::L4440 Plasmid Data

Plasmid 11336: lis-1::L4440

Gene/insert name:
lis-1
Insert size:
1200
Species:
C. elegans (nematode)
GenBank ID:
NM_067354
Entrez Gene:
lis-1 ()
Vector backbone:
L4440
(Search Vector Database)
Backbone manufacturer:
L4440 is available at Addgene
Vector type:
Worm Expression, RNAi
Backbone size w/o insert (bp):
2790
5' sequencing primer:
L4440 primer (AGCGAGTCAGTGAGCGAG) List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Dr. Andrew Fire created the vector backbone L4440
Sequence: View sequences (2)
Principal Investigator:
Guy Caldwell
Terms and Licenses:
MTA

Comments:

Full length cDNA for C. elegans open-reading frame T03F6.5, encoding the lis-1 gene, cloned into RNAi feeding vector L4440. Can be used for RNAi feeding; should result in embryonic lethality at high levels of induction.

This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11336" in your Materials and Methods section.