Addgene - GFP::L4440 Plasmid Data

Plasmid 11335: GFP::L4440

Gene/insert name:
GFP
Alt name:
green fluorescence protein
Insert size:
869
Vector backbone:
L4440
(Search Vector Database)
Backbone manufacturer:
L4440 is available at Addgene
Vector type:
Worm Expression, RNAi
Backbone size w/o insert (bp):
2790
5' sequencing primer:
L4440 primer (AGCGAGTCAGTGAGCGAG) List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Dr. Andrew Fire created the vector backbone L4440 and the GFP sequence in vector p95.77
Sequence: View sequences (1)
Principal Investigator:
Guy Caldwell
Terms and Licenses:
MTA

Comments:

Full-length GFP sequence (from vector p95.77) was cloned into RNAi feeding vector L4440. Use in a strain carrying a GFP construct to observe reduction of GFP signal after RNAi induction.

This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11335" in your Materials and Methods section.