Addgene - pLexA::lis-1 Plasmid Data

Plasmid 11338: pLexA::lis-1

Gene/insert name:
lis-1
Insert size:
1200
Species:
C. elegans (nematode)
GenBank ID:
NM_067354
Entrez Gene:
lis-1 ()
Fusion protein or tag:
LexA DNA Binding Domain
Terminal:
N terminal on backbone
Vector backbone:
pLexA
(Search Vector Database)
Backbone manufacturer:
see pLexA map in "author's map"
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
5700
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
PstI
Site destroyed during cloning:
No
5' sequencing primer:
LexA sequencing primer (CGTCAGCAGAGCTTCACCATTG) List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
TRP1
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Guy Caldwell
Terms and Licenses:
MTA

Comments:

Full-length cDNA for C. elegans open-reading frame T03F6.5, encoding the lis-1 gene, cloned into the yeast two-hybrid DNA-binding domain vector pLexA. This vector can be used as the bait for a yeast two-hybrid screen. See "author's map" for picture of empty pLexA vector.

This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11338" in your Materials and Methods section.