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Fire Lab C. elegans Vector Kit Icon

Fire Lab C. elegans Vector Kit
(Kit # 1000000001 )

Depositing Labs:   Andrew Fire

Dr. Andrew Fire's lab has deposited a set of vectors for C. elegans research. This kit contains 288 vectors and includes both a general description and the full predicted DNA sequence for each vector.

This kit consists of 3 plates (288 plasmids), and will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs. Addgene does not sell worm strains.

Add to Cart
$375 USD + shipping

Available to academics and nonprofits only.

Description

Dr. Andrew Fire's lab has deposited a set of vectors for C. elegans research. This kit contains 288 vectors and includes both a general description and the full predicted DNA sequence for each vector. Among these vectors are:

  • lacZ and/or GFP fusion vectors with higher expression efficiency than many earlier C. elegans vectors. These include vectors with coding region for blue-shifted GFP, cyan-shifted GFP, yellow-shifted GFP, and other GFP protein variants.

  • Vectors for tagging C. elegans exons and introns with GFP.

  • Control plasmids for RNA-mediated interference.

  • Hairpin sequences to test promoters for ability to trigger RNAi in-vivo.

Kit Documentation

Icon Fire Lab C. elegans Vector Kit Info Addgene (238.8 KB)


The vectors in this kit are divided into 3 plates (96 samples each). The sample plate location information and documentaion are as follows:

Additional documents from the Fire lab:

More Downloadable Documentation

In these kit documentations, plasmids may be referred by their mini-prep number (prefix pPD), or ligation number (prefix L). Please consult the Excel file below for translation between these naming schemes. In the Addgene library, you may search for a plasmid using either number.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The Fire Lab C. elegans Vector Kit was a gift from Andrew Fire (Addgene kit # 1000000001)"

Note that Plate 1 listed on this page corresponds to Fire Lab C. Elegans Vector Kit 1995, Plate 2 corresponds to Kit 1997 and Plate 3 corresponds to Kit 1999

Fire Lab C. Elegans Kit - #1000000001

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin
Kanamycin

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 pPD87_18
Ampicillin
Plate 1 / A / 2 pPD90_23
Ampicillin
Plate 1 / A / 3 pPD95_67
Ampicillin
Plate 1 / A / 4 pPD80_08
Ampicillin
Plate 1 / A / 5 pPD88_22
Ampicillin
Plate 1 / A / 6 pPD90_28
Ampicillin
Plate 1 / A / 7 pPD95_70
Ampicillin
Plate 1 / A / 8 pPD95_93
Ampicillin
Plate 1 / A / 9 pPD87_50
Ampicillin
Plate 1 / A / 10 pPD90_31
Ampicillin
Plate 1 / A / 11 pPD95_69
Ampicillin
Plate 1 / A / 12 pPD96_02
Ampicillin
Plate 1 / B / 1 pPD88_27
Ampicillin
Plate 1 / B / 2 pPD89_03
Ampicillin
Plate 1 / B / 3 pPD95_73
Ampicillin
Plate 1 / B / 4 pPD96_04
Ampicillin
Plate 1 / B / 5 pPD91_14
Ampicillin
Plate 1 / B / 6 pPD89_09
Ampicillin
Plate 1 / B / 7 pPD95_79
Ampicillin
Plate 1 / B / 8 pOK11_34
Ampicillin
Plate 1 / B / 9 pPD95_15
Ampicillin
Plate 1 / B / 10 pPD89_20
Ampicillin
Plate 1 / B / 11 pPD95_77
Ampicillin
Plate 1 / B / 12 pPD95_62
Ampicillin
Plate 1 / C / 1 pPD88_75
Ampicillin
Plate 1 / C / 2 pPD89_17
Ampicillin
Plate 1 / C / 3 pPD95_75
Ampicillin
Plate 1 / C / 4 pPD96_62
Ampicillin
Plate 1 / C / 5 pPD96_12
Ampicillin
Plate 1 / C / 6 pPD95_03
Ampicillin
Plate 1 / C / 7 pPD95_81
Ampicillin
Plate 1 / C / 8 pPD69_39
Ampicillin
Plate 1 / C / 9 pPD93_51
Ampicillin
Plate 1 / C / 10 pPD95_07
Ampicillin
Plate 1 / C / 11 pPD95_85
Ampicillin
Plate 1 / C / 12 pPD95_16
Ampicillin
Plate 1 / D / 1 pPD93_48
Ampicillin
Plate 1 / D / 2 pPD95_57
Ampicillin
Plate 1 / D / 3 pPD96_32
Ampicillin
Plate 1 / D / 4 pPD95_21
Ampicillin
Plate 1 / D / 5 pPD93_65
Ampicillin
Plate 1 / D / 6 pPD95_10
Ampicillin
Plate 1 / D / 7 pPD79_44
Ampicillin
Plate 1 / D / 8 pPD95_25
Ampicillin
Plate 1 / D / 9 pPD94_81
Ampicillin
Plate 1 / D / 10 pPD95_11
Ampicillin
Plate 1 / D / 11 pPD95_02
Ampicillin
Plate 1 / D / 12 pPD95_27
Ampicillin
Plate 1 / E / 1 pPD47_52
Kanamycin
Plate 1 / E / 2 pPD10_46
Ampicillin
Plate 1 / E / 3 pPD50_21
Ampicillin
Plate 1 / E / 4 pICT19h
Ampicillin
Plate 1 / E / 5 pPD57_56
Ampicillin
Plate 1 / E / 6 pPD26_77
Ampicillin
Plate 1 / E / 7 pPD20_97
Ampicillin
Plate 1 / E / 8 pAST_19a
Ampicillin
Plate 1 / E / 9 pPD8_02
Ampicillin
Plate 1 / E / 10 pPD34_110
Ampicillin
Plate 1 / E / 11 pPD50_14
Ampicillin
Plate 1 / E / 12 pAST_18b
Ampicillin
Plate 1 / F / 1 pPD8_33
Ampicillin
Plate 1 / F / 2 pPD81_34
Ampicillin
Plate 1 / F / 3 pPD49_78
Ampicillin
Plate 1 / F / 4 pPD16_43
Ampicillin
Plate 1 / F / 5 pPD61_125
Ampicillin
Plate 1 / F / 6 pPD49_83
Ampicillin
Plate 1 / F / 7 pPD16_51
Ampicillin
Plate 1 / F / 8 pPD93_97
Ampicillin
Plate 1 / F / 9 pPD30_69
Ampicillin
Plate 1 / F / 10 pPD95_18
Ampicillin
Plate 1 / F / 11 pPD52_37
Ampicillin
Plate 1 / F / 12 pPD18_32
Ampicillin
Plate 1 / G / 1 pPD95_86
Ampicillin
Plate 1 / G / 2 pPD54_01
Ampicillin
Plate 1 / G / 3 pPD21_28
Ampicillin
Plate 1 / G / 4 pPD5_41
Ampicillin
Plate 1 / G / 5 pPD52_102
Ampicillin
Plate 1 / G / 6 pPD22_11
Ampicillin
Plate 1 / G / 7 pPD30_35
Ampicillin
Plate 1 / G / 8 pPD22_04
Ampicillin
Plate 1 / G / 9 pICT19r
Ampicillin
Plate 1 / G / 10 pPD18_49
Ampicillin
Plate 1 / G / 11 pPD30_38
Ampicillin
Plate 1 / G / 12 pDD16_01
Ampicillin
Plate 1 / H / 1 pPD152.79
Ampicillin
Plate 1 / H / 2 pPD157.60
Ampicillin
Plate 1 / H / 3 pPD160.60
Ampicillin
Plate 1 / H / 4 pPD158.87
Ampicillin
Plate 1 / H / 5 pPD157.63
Ampicillin
Plate 1 / H / 6 pPD117.20
Ampicillin
Plate 1 / H / 7 pPD157.99
Ampicillin
Plate 1 / H / 8 pPD138.11
Ampicillin
Plate 1 / H / 9 pPD132.49
Ampicillin
Plate 1 / H / 10 pPD172.01
Ampicillin
Plate 1 / H / 11 pPD176.36
Ampicillin
Plate 1 / H / 12 pPD176.86
Ampicillin
Plate 2 / A / 1 L2603
Ampicillin
Plate 2 / A / 2 L3567
Ampicillin
Plate 2 / A / 3 L2604
Ampicillin
Plate 2 / A / 4 L3568
Ampicillin
Plate 2 / A / 5 L2605
Ampicillin
Plate 2 / A / 6 L3569
Ampicillin
Plate 2 / A / 7 L2630
Ampicillin
Plate 2 / A / 8 L3570
Ampicillin
Plate 2 / A / 9 L2678
Ampicillin
Plate 2 / A / 10 L3571
Ampicillin
Plate 2 / A / 11 L2680
Ampicillin
Plate 2 / A / 12 L3572
Ampicillin
Plate 2 / B / 1 L3573
Ampicillin
Plate 2 / B / 2 L2681
Ampicillin
Plate 2 / B / 3 L3574
Ampicillin
Plate 2 / B / 4 L2682
Ampicillin
Plate 2 / B / 5 L3602
Ampicillin
Plate 2 / B / 6 L2684
Ampicillin
Plate 2 / B / 7 L3603
Ampicillin
Plate 2 / B / 8 L2685
Ampicillin
Plate 2 / B / 9 L3604
Ampicillin
Plate 2 / B / 10 L2822
Ampicillin
Plate 2 / B / 11 L3605
Ampicillin
Plate 2 / B / 12 L2865
Ampicillin
Plate 2 / C / 1 L2908
Ampicillin
Plate 2 / C / 2 L3606
Ampicillin
Plate 2 / C / 3 L2911
Ampicillin
Plate 2 / C / 4 L3607
Ampicillin
Plate 2 / C / 5 L2944
Ampicillin
Plate 2 / C / 6 L3608
Ampicillin
Plate 2 / C / 7 L2946
Ampicillin
Plate 2 / C / 8 L3609
Ampicillin
Plate 2 / C / 9 L2947
Ampicillin
Plate 2 / C / 10 L3610
Ampicillin
Plate 2 / C / 11 L2963
Ampicillin
Plate 2 / C / 12 L3611
Ampicillin
Plate 2 / D / 1 L3612
Ampicillin
Plate 2 / D / 2 L3110
Ampicillin
Plate 2 / D / 3 L3613
Ampicillin
Plate 2 / D / 4 L3111
Ampicillin
Plate 2 / D / 5 L3615
Ampicillin
Plate 2 / D / 6 L3135
Ampicillin
Plate 2 / D / 7 L3616
Ampicillin
Plate 2 / D / 8 L3136
Ampicillin
Plate 2 / D / 9 L3617
Ampicillin
Plate 2 / D / 10 L3137
Ampicillin
Plate 2 / D / 11 L3618
Ampicillin
Plate 2 / D / 12 L3465
Ampicillin
Plate 2 / E / 1 L3467
Ampicillin
Plate 2 / E / 2 L3619
Ampicillin
Plate 2 / E / 3 L3469
Ampicillin
Plate 2 / E / 4 L3620
Ampicillin
Plate 2 / E / 5 L3471
Ampicillin
Plate 2 / E / 6 L3621
Ampicillin
Plate 2 / E / 7 L3502
Ampicillin
Plate 2 / E / 8 L3691
Ampicillin
Plate 2 / E / 9 L3503
Ampicillin
Plate 2 / E / 10 L3774
Ampicillin
Plate 2 / E / 11 L3505
Ampicillin
Plate 2 / E / 12 L3781
Ampicillin
Plate 2 / F / 1 L3784
Ampicillin
Plate 2 / F / 2 L3506
Ampicillin
Plate 2 / F / 3 L3785
Ampicillin
Plate 2 / F / 4 L3513
Ampicillin
Plate 2 / F / 5 L3786
Ampicillin
Plate 2 / F / 6 L3514
Ampicillin
Plate 2 / F / 7 L3787
Ampicillin
Plate 2 / F / 8 L3515
Ampicillin
Plate 2 / F / 9 L3788
Ampicillin
Plate 2 / F / 10 L3516
Ampicillin
Plate 2 / F / 11 L3790
Ampicillin
Plate 2 / F / 12 L3517
Ampicillin
Plate 2 / G / 1 L3522
Ampicillin
Plate 2 / G / 2 L3812
Ampicillin
Plate 2 / G / 3 L3527
Ampicillin
Plate 2 / G / 4 L3799
Ampicillin
Plate 2 / G / 5 L3557
Ampicillin
Plate 2 / G / 6 L3827
Ampicillin
Plate 2 / G / 7 L3558
Ampicillin
Plate 2 / G / 8 L3828
Ampicillin
Plate 2 / G / 9 L3559
Ampicillin
Plate 2 / G / 10 L3829
Ampicillin
Plate 2 / G / 11 L3560
Ampicillin
Plate 2 / G / 12 L3863
Ampicillin
Plate 2 / H / 1 L3809
Ampicillin
Plate 2 / H / 2 L3561
Ampicillin
Plate 2 / H / 3 L3670
Ampicillin
Plate 2 / H / 4 L3562
Ampicillin
Plate 2 / H / 5 L3808
Ampicillin
Plate 2 / H / 6 L3563
Ampicillin
Plate 2 / H / 7 L3811
Ampicillin
Plate 2 / H / 8 L3564
Ampicillin
Plate 2 / H / 9 L3720
Ampicillin
Plate 2 / H / 10 L3565
Ampicillin
Plate 2 / H / 11 BK48
Ampicillin
Plate 2 / H / 12 L3566
Ampicillin
Plate 3 / A / 1 L4816
Ampicillin
Plate 3 / A / 2 L4053
Ampicillin
Plate 3 / A / 3 L4663
Ampicillin
Plate 3 / A / 4 L4051
Ampicillin
Plate 3 / A / 5 L4686
Ampicillin
Plate 3 / A / 6 L4040
Ampicillin
Plate 3 / A / 7 L4665
Ampicillin
Plate 3 / A / 8 L4037
Ampicillin
Plate 3 / A / 9 L4664
Ampicillin
Plate 3 / A / 10 L4042
Ampicillin
Plate 3 / A / 11 L4682
Ampicillin
Plate 3 / A / 12 L4043
Ampicillin
Plate 3 / B / 1 L4038
Ampicillin
Plate 3 / B / 2 L4660
Ampicillin
Plate 3 / B / 3 L4453
Ampicillin
Plate 3 / B / 4 L4780
Ampicillin
Plate 3 / B / 5 L4455
Ampicillin
Plate 3 / B / 6 L4752
Ampicillin
Plate 3 / B / 7 L4759
Ampicillin
Plate 3 / B / 8 L4817
Ampicillin
Plate 3 / B / 9 L4796
Ampicillin
Plate 3 / B / 10 L4643
Ampicillin
Plate 3 / B / 11 L4809
Ampicillin
Plate 3 / B / 12 L4687
Ampicillin
Plate 3 / C / 1 L4667
Ampicillin
Plate 3 / C / 2 L4057
Ampicillin
Plate 3 / C / 3 L4666
Ampicillin
Plate 3 / C / 4 L4058
Ampicillin
Plate 3 / C / 5 L4683
Ampicillin
Plate 3 / C / 6 L4035
Ampicillin
Plate 3 / C / 7 L4671
Ampicillin
Plate 3 / C / 8 L453
Ampicillin
Plate 3 / C / 9 L4781
Ampicillin
Plate 3 / C / 10 L4129
Ampicillin
Plate 3 / C / 11 L4753
Ampicillin
Plate 3 / C / 12 L4119
Ampicillin
Plate 3 / D / 1 L4138
Ampicillin
Plate 3 / D / 2 L4640
Ampicillin
Plate 3 / D / 3 L4819
Ampicillin
Plate 3 / D / 4 L4061
Ampicillin
Plate 3 / D / 5 L4440
Ampicillin
Plate 3 / D / 6 L4062
Ampicillin
Plate 3 / D / 7 pLT61
Ampicillin
Plate 3 / D / 8 L4063
Ampicillin
Plate 3 / D / 9 pLT63
Ampicillin
Plate 3 / D / 10 L4064
Ampicillin
Plate 3 / D / 11 L4417
Ampicillin
Plate 3 / D / 12 L4065
Ampicillin
Plate 3 / E / 1 L4066
Ampicillin
Plate 3 / E / 2 L4400
Ampicillin
Plate 3 / E / 3 L4095
Ampicillin
Plate 3 / E / 4 L4271
Ampicillin
Plate 3 / E / 5 L4096
Ampicillin
Plate 3 / E / 6 L4404
Ampicillin
Plate 3 / E / 7 L4458
Ampicillin
Plate 3 / E / 8 pPD191.45
Ampicillin
Plate 3 / E / 9 L4506
Ampicillin
Plate 3 / E / 10 L4431
Ampicillin
Plate 3 / E / 11 L4507
Ampicillin
Plate 3 / E / 12 L4842
Ampicillin
Plate 3 / F / 1 L4437
Ampicillin
Plate 3 / F / 2 L4632
Ampicillin
Plate 3 / F / 3 L4438
Ampicillin
Plate 3 / F / 4 L4054
Ampicillin
Plate 3 / F / 5 pBK125
Ampicillin
Plate 3 / F / 6 L4052
Ampicillin
Plate 3 / F / 7 pPD187.49
Ampicillin
Plate 3 / F / 8 L4019
Ampicillin
Plate 3 / F / 9 L754
Ampicillin
Plate 3 / F / 10 L4047
Ampicillin
Plate 3 / F / 11 L2528
Ampicillin
Plate 3 / F / 12 L4018
Ampicillin
Plate 3 / G / 1 L4046
Ampicillin
Plate 3 / G / 2 L2534
Ampicillin
Plate 3 / G / 3 L4020
Ampicillin
Plate 3 / G / 4 L2531
Ampicillin
Plate 3 / G / 5 L4048
Ampicillin
Plate 3 / G / 6 L3929
Ampicillin
Plate 3 / G / 7 L4041
Ampicillin
Plate 3 / G / 8 L3928
Ampicillin
Plate 3 / G / 9 L4026
Ampicillin
Plate 3 / G / 10 L3930
Ampicillin
Plate 3 / G / 11 L4049
Ampicillin
Plate 3 / G / 12 L3920
Ampicillin
Plate 3 / H / 1 L3927
Ampicillin
Plate 3 / H / 2 pJKL464
Ampicillin
Plate 3 / H / 3 pPD177.53
Ampicillin
Plate 3 / H / 4 pPD177.62
Ampicillin
Plate 3 / H / 5 pPD177.66
Ampicillin
Plate 3 / H / 6 pPD177.71
Ampicillin
Plate 3 / H / 7 pPD187.24
Ampicillin
Plate 3 / H / 8 pPD159.88
Ampicillin
Plate 3 / H / 9 pPD107.09
Ampicillin
Plate 3 / H / 10 pPD158.114
Ampicillin
Plate 3 / H / 11 pPD158.117
Ampicillin
Plate 3 / H / 12 pPD159.62
Ampicillin
Data calculated @ 2016-12-09

Kit Plate Map - #1000000001

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