This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

plasmid kit icon

SapTrap CRISPR/Cas Toolkit
(Kit # 1000000077 )

Depositing Lab:   Erik Jorgensen

The SapTrap Toolkit is a collection of plasmids that streamlines the production of targeting vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans strains.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$350 USD + shipping

Available to academics and nonprofits only.

Original Publication

SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans. Schwartz ML, Jorgensen EM. Genetics. 2016 Feb 2. pii: genetics.115.184275. [Epub ahead of print] PubMed PMID: 26837755 .

Description

SapTrap is a modular toolkit for building CRISPR targeting vectors to insert genetic tags in the C. elegans genome. The SapTrap reaction produces a single plasmid targeting vector that encodes both a guide RNA transcript and a repair template for an individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt Unc-32::GFP targeting vector.

jorgensen saptrap kit diagram
Plasmid Assembly Pipeline. Figure S2 from Schwartz et al, 2016. PubMed PMID: 26837755 . Courtesy of Genetics Society of America.

Depositor-provided Files

The Jorgensen Lab has provided a methods file that includes information about:

  • Oligo design tips and explanatory figures for SapTrap vectors
  • SapTrap assembly procedure
  • Protocol for injection and isolation
Icon Jorgensen-SapTrapKit-Protocols (4.1 MB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The SapTrap kit was a gift from Erik Jorgensen (Addgene kit # 1000000077).”

For your Reference section:

SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans. Schwartz ML, Jorgensen EM. Genetics. 2016 Feb 2. pii: genetics.115.184275. [Epub ahead of print] PubMed PMID: 26837755 .

SapTrap Kit - #1000000077

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin
Kanamycin

Inventory

Well Plasmid Resistance
A / 1 pMLS134
Ampicillin
A / 2 pMLS256
Ampicillin
A / 3 pMLS257
Ampicillin
A / 4 pMLS328
Ampicillin
A / 5 pMLS262
Ampicillin
A / 6 pMLS338
Ampicillin
A / 7 pMLS252
Kanamycin
A / 8 pMLS254
Kanamycin
A / 9 pMLS271
Kanamycin
A / 10 pMLS286
Kanamycin
A / 11 pMLS291
Kanamycin
A / 12 pMLS292
Kanamycin
B / 1 pMLS268
Kanamycin
B / 2 pMLS269
Kanamycin
B / 3 pMLS272
Kanamycin
B / 4 pMLS279
Kanamycin
B / 5 pMLS287
Kanamycin
B / 6 pMLS382
Kanamycin
B / 7 pMLS281
Kanamycin
B / 8 pMLS282
Kanamycin
B / 9 pMLS285
Kanamycin
B / 10 pMLS288
Kanamycin
B / 11 pMLS383
Kanamycin
B / 12 pMLS297
Kanamycin
C / 1 pMLS280
Kanamycin
C / 2 pWD509
Kanamycin
Data calculated @ 2017-08-17

Kit Plate Map - #1000000077

Plate #1

  1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
Content blocked, you may need to disable your ad-blocker.