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Sybody Generation Toolbox
(Kit # 1000000160 )

Depositing Lab:   Markus Seeger

The Seeger Lab has established a protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation process.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$330 USD + shipping

Available to academics and nonprofits only.

Original Publications

Generation of Synthetic Nanobodies Against Delicate Proteins. Zimmermann I, Egloff P, Hutter CAJ, Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA. Nature Protocols. 2020 Apr 8. doi: 10.1038/s41596-020-0304-x. PubMed PMID: 32269381.

Synthetic Single Domain Antibodies for the Conformational Trapping of Membrane Proteins. Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA. Elife. 2018 May 24;7. pii: 34317. doi: 10.7554/eLife.34317. PubMed PMID: 29792401.

Description

The Seeger Lab has established a general protocol to select synthetic nanobodies – called sybodies – against soluble proteins, membrane proteins, and protein complexes. This collection contains all plasmids necessary for the entirely in vitro sybody generation process. A detailed protocol referencing all of the plasmids can be found in the Nature Protocols article.

Schematic overview of Sybody generation
  • Schematic overview of the sybody platform. Sybodies against protein targets can be generated within three weeks.

Protocols

Generation of Synthetic Nanobodies Against Delicate Proteins. Zimmermann I, Egloff P, Hutter CAJ, Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA. Nature Protocols. 2020 Apr 8. doi: 10.1038/s41596-020-0304-x. PubMed PMID: 32269381.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The Sybody Generation Toolbox was a gift from Markus Seeger (Addgene kit #1000000160).”

For your Reference section:

Generation of Synthetic Nanobodies Against Delicate Proteins. Zimmermann I, Egloff P, Hutter CAJ, Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA. Nature Protocols. 2020 Apr 8. doi: 10.1038/s41596-020-0304-x. PubMed PMID: 32269381.

Synthetic Single Domain Antibodies for the Conformational Trapping of Membrane Proteins. Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA. Elife. 2018 May 24;7. pii: 34317. doi: 10.7554/eLife.34317. PubMed PMID: 29792401.

Sybody Generation Toolbox - #1000000160

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Chloramphenicol and Ampicillin
Chloramphenicol
Ampicillin

Inventory

Well Plasmid Resistance
A / 1 pBXNPH3
Chloramphenicol and Ampicillin
A / 2 pBXNPHM3
Chloramphenicol and Ampicillin
A / 3 pSBinit
Chloramphenicol
A / 4 pSb_init containing the TM287/288 nanobody Nb_TM#2
Chloramphenicol
A / 5 pSb_init containing the MBP sybody Sb_MBP#1
Chloramphenicol
A / 6 pDXinit
Ampicillin
A / 7 SB_concave
Ampicillin
A / 8 SB_loop
Ampicillin
A / 9 SB_convex
Ampicillin
A / 10 pRDV5 containing Sb-loop
Ampicillin
A / 11 pRDV5 containing Sb-convex
Ampicillin
A / 12 pDX_init containing Sb-loop
Ampicillin
B / 1 pBXNH3CA_MBP
Ampicillin
B / 2 pBXNH3LCA_TM287/288
Ampicillin
Data calculated @ 2020-09-20

Kit Plate Map - #1000000160

Plate #1

  1 2 3 4 5 6 7 8 9 10 11 12
A
B
C
D
E
F
G
H
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