Addgene - pSuper mouse DGCR8-1 Plasmid Data

Gene/insert name:		DGCR8 shRNA
Alternative names:		DGCR8
Insert size (bp):		Unknown
Gene/insert aliases:		Dgcr8, Gy1, N41, D16Wis2, Vo59c07, D16H22S788E, D16H22S1742E
Species of gene(s):		M. musculus (mouse)
Vector backbone:		pSUPER.retro.puro (Search Vector Database)
Backbone manufacturer:		OligoEngine
Type of vector:		Mammalian expression,Retroviral,RNAi
Backbone size (bp):		6350
5' Sequencing primer:		pLXSN5' (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Puromycin
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Tyler Jacks
Terms and Licenses:		MTA

Comments: shRNA sequence: GATCCCCCCA ATGATGACCA AGATTATTCA AGAGATAATC TTGGTCATCA TTGGTTTTTG GAAC

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Impaired microRNA processing enhances cellular transformation and tumorigenesis. Kumar MS et al. (Nat Genet. 2007 Apr 1. ():. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 14772" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.