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Green monkey (Chlorocebus sabaeus) sgRNA library
(Pooled Library #178284)

  • Purpose

    This lentiviral genome-wide CRISPR knockout library targets the green monkey (Chlorocebus sabaeus, txid: 60711) genome with 4 sgRNA per gene.

  • Vector Backbone

    pXPR_050 - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 178284 Green monkey sgRNA library 1 $600 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pLX_311-Cas9 (Addgene #96924) or any other plasmid(s) or cell lines expressing Cas9.

Library Details

  • Species
    Green monkey (Chlorocebus sabaeus)
  • Genes targeted
    20,991
  • gRNAs
    84,963
  • Controls
    1,000
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    100ng/µL
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Green monkey (Chlorocebus sabaeus) sgRNA library was a gift from John Doench and David Root (Addgene #178284)
  • For your References section:

    Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. Wei J, Alfajaro MM, DeWeirdt PC, Hanna RE, Lu-Culligan WJ, Cai WL, Strine MS, Zhang SM, Graziano VR, Schmitz CO, Chen JS, Mankowski MC, Filler RB, Ravindra NG, Gasque V, de Miguel FJ, Patil A, Chen H, Oguntuyo KY, Abriola L, Surovtseva YV, Orchard RC, Lee B, Lindenbach BD, Politi K, van Dijk D, Kadoch C, Simon MD, Yan Q, Doench JG, Wilen CB. Cell. 2021 Jan 7;184(1):76-91.e13. doi: 10.1016/j.cell.2020.10.028. PubMed 33147444