- Purpose
This library is intended to be used as a technical tool for removal of mitochondrial DNA from mouse genomic DNA preparations.
This genome engineering process is called “CRISPR/Cas9-assisted removal of mitochondrial DNA (CARM)”.
- Vector Backbone
pUC57kan-T7 - this backbone does not contain Cas9
- Depositing Lab
- Publication
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 82480 | Mouse gRNA pooled sub-library targeting mitochondrial DNA (pUC57kan-T7-mt-gRNA)† | 1 | $ 540 | Add to Cart | |
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately.
Library Details
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SpeciesMouse
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Genes targetedgRNAs target every ∼40bp over mitochondrial genome
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gRNAs395
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ControlsN/A
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Lentiviral GenerationN/A
Library Shipping
This library will be delivered as a pooled DNA library in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.
You will receive:
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Volume∼10µL
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Concentration10ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:
pUC57kan-T7-mt-gRNA Library was a gift from Wei Xie (Addgene # 82480).For your References section:
The landscape of accessible chromatin in mammalian preimplantation embryos. Wu J, Huang B, Chen H, Yin Q, Liu Y, Xiang Y, Zhang B, Liu B, Wang Q, Xia W, Li W, Li Y, Ma J, Peng X, Zheng H, Ming J, Zhang W, Zhang J, Tian G, Xu F, Chang Z, Na J, Yang X, Xie W. Nature. 2016 Jun 30;534(7609):652-7. PubMed 27309802