Holiday Schedule: Addgene will be closed December 22nd & 25th and December 29th & January 1st. For details, see our holiday shipping schedule. If you have any questions, please contact us at [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Human Improved Genome-wide Knockout CRISPR Library
(Pooled Library #67989)

  • Purpose

    A gRNA library against 18,010 human genes, designed for use in lentivirus.

    gRNAs were redesigned using a new design pipeline as well as an improved scaffold.

  • Vector Backbone

    pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 67989
  • gRNA pooled library

1 $500 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pKLV2-EF1a-Cas9Bsd-W (Addgene #68343) or with cell lines already expressing Cas9.

Library Details

  • Species
    Human
  • Genes targeted
    18,010
  • gRNAs
    90,709
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼50µL
  • Concentration
    20ng/µL

Depositor Comments

This library consists of 90,709 guide sequences targeting a total of 18,010 human genes.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    Human Improved Genome-wide Knockout CRISPR Library v1 was a gift from Kosuke Yusa (Addgene #67989)
  • For your References section:

    A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly BJ, Iorio F, Pina C, Vassiliou GS, Yusa K. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079PubMed 27760321