Showing: 1 - 20 of 26471 results
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Plasmid#154756PurposeFor curing of the resistance marker of RecET recombinase system in Acinetobacter baumannii, AprarDepositorInsertFLP recombinase
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-His-SCov2-nsp7
Plasmid#154757PurposeExpression of SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET-His-SCov2-nsp8
Plasmid#154758PurposeExpression of SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pASPIre1
Plasmid#154842PurposeDerivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sitesDepositorInsertBxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
UseTagsExpressionBacterialMutationPromoterPrhaAvailabilityAcademic Institutions and Nonprofits only -
pASPIre2
Plasmid#154843PurposeDerivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1DepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pASPIre3
Plasmid#154844PurposeDerivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this studyDepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-pgaC
Plasmid#154919PurposeaTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymesDepositorInsertgRNA targeting pgaC
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-pheT
Plasmid#154920PurposeaTc inducible gRNA targeting pheT along with arabinose-inducible lambda red enzymesDepositorInsertgRNA targeting pheT
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-mqo
Plasmid#154921PurposeaTc inducible gRNA targeting mqo along with arabinose-inducible lambda red enzymesDepositorInsertgRNA targeting mqo
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-hisF
Plasmid#154922PurposeaTc inducible gRNA targeting hisF along with arabinose-inducible lambda red enzymesDepositorInsertgRNA targeting hisF
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-ampG
Plasmid#154923PurposeaTc inducible gRNA targeting ampG along with arabinose-inducible lambda red enzymesDepositorInsertgRNA targeting ampG
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-D1135E
Plasmid#154924PurposeaTc inducible Cas9HF1 with D1135E mutationDepositorInsertCas9HF1-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationN497A/R661A/Q695A/Q926A/D1135E and stop codon on …PromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pEM-eCas9
Plasmid#154925PurposeaTc inducible eCas9DepositorInserteCas9
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationK848A, K1003A, R1060APromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pEM-eCas9-D1135E
Plasmid#154926PurposeaTc inducible eCas9 with D1135E mutationDepositorInserteCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationK848A, K1003A, R1060A, D1135EPromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pEM-OptiCas9
Plasmid#154927PurposeaTc inducible OptiCas9DepositorInsertOptiCas9
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationR661A, K1003HPromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pEM-OptiCas9-D1135E
Plasmid#154928PurposeaTc inducible OptiCas9 with D1135E mutationDepositorInsertOptiCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationR661A, K1003H, D1135EPromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pEM-HypaCas9
Plasmid#154929PurposeaTc inducible HypaCas9DepositorInsertHypaCas9
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationN692A/M694A/Q695A/H698APromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pEM-HypaCas9-D1135E
Plasmid#154930PurposeaTc inducible HypaCas9 with D1135E mutationDepositorInsertHypaCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationN692A/M694A/Q695A/H698A/D1135EPromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9-D1135E
Plasmid#154931PurposeaTc inducible Cas9 with D1135E mutationDepositorInsertCas9-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationD1135EPromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pNT-15
Plasmid#154932Purpose15 designed non-target sitesDepositorInsert15 designed non-target sites
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 26471 results