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Plasmid#158538PurposeBacterial expression of rhomboid protease HiGlpGDepositorInsertHiGlpG
UseTagsGST tagExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HiGlpG- S116A, H196A
Plasmid#158539PurposeBacterial expression of rhomboid protease catalytic mutant HiGlpG-S116A,H196ADepositorInsertHiGlpG
UseTagsGST tagExpressionBacterialMutationS116A;H169APromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
SnapTag-Binder
Plasmid#179142PurposeBinder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).DepositorInsertSspB
UseTagsSNAP-tagExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9PromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-mCherry-Binder
Plasmid#179139PurposeBinder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).DepositorInsertSspB
UseTagsFLAG and mCherryExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9PromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
yPet_cp229-Binder
Plasmid#179137PurposeYPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9).DepositorInsertSspB
UseTagsyPet_cp229ExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9PromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Negative Control Binder
Plasmid#179140PurposeBinder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9) and an additionional A70Q mutation greatly reduces affinity for SsrA peptideDepositorInsertSspB
UseTagsFLAG and mCherryExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9. Addi…PromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
prhaBAD-pA-Hia5
Plasmid#222303PurposePlasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertpA-Hia5
UseTags6His and protein AExpressionBacterialMutationcodon optimized for bacterial expressionPromoterrhaBADAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS1026-pMCSG7-HpaCas9
Plasmid#121540PurposeExpresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cellsDepositorInsertHpaCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS540-pCSDest2-AcrIIC4Hpa
Plasmid#113434PurposeExpresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cellsDepositorInsertType II-C anti-CRISPR
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS
Plasmid#113436PurposeExpresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cellsDepositorInserttype II-C anti-CRISPR
UseCRISPRTagsFLAG/NLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS663-pMCSG7-AcrIIC4Hpa
Plasmid#113440PurposeExpresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cellsDepositorInsertType II-C anti-CRISPR with N-terminal 6xHis and TEV site
UseTags6xHisExpressionBacterialMutationPromoterlacIAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS592-pCSDest2-AcrIIC4Hpa-BFPv2-IRES
Plasmid#113438PurposeExpresses type II-C anti-CRISPR protein from H. parainfluenzae and a BFP from IRES in mammalian cellsDepositorInsertsType II-C anti-CRISPR
mTagBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only