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Showing: 41 - 60 of 839 results

  2. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Expression
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Availability
    Academic Institutions and Nonprofits only
    171090-plasmid-map-sequence-id-335786

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  3. pFG001

    Plasmid
    #62816
    Purpose
    Bacterial constitutive gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PN25
    Availability
    Academic Institutions and Nonprofits only
    62816-plasmid-map-sequence-id-123824

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  4. pFG018

    Plasmid
    #62817
    Purpose
    Bacterial inducible gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Availability
    Academic Institutions and Nonprofits only
    62817-plasmid-map-sequence-id-114422

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  5. pCMK

    Plasmid
    #62818
    Purpose
    Bacterial constitutive S. pyogenes Cas9 expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    62818-plasmid-map-sequence-id-114423

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  6. AA025

    Plasmid
    #216005
    Purpose
    Fragmid fragment: (Cas protein) NG PAM
    Depositor
    John Doench
    Insert
    Cas9-NG_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216005-plasmid-map-sequence-id-431447

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  7. AA026

    Plasmid
    #216006
    Purpose
    Fragmid fragment: (Cas protein) NG PAM
    Depositor
    John Doench
    Insert
    Cas9-SpG_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216006-plasmid-map-sequence-id-431428

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  8. AA032

    Plasmid
    #216012
    Purpose
    Fragmid fragment: (Cas protein) deactivated Cas
    Depositor
    John Doench
    Insert
    dCas9_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216012-plasmid-map-sequence-id-431460

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  9. AA033

    Plasmid
    #216013
    Purpose
    Fragmid fragment: (Cas protein) deactivated Cas
    Depositor
    John Doench
    Insert
    dCas9-NG_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216013-plasmid-map-sequence-id-431529

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  10. AA034

    Plasmid
    #216014
    Purpose
    Fragmid fragment: (Cas protein) deactivated Cas
    Depositor
    John Doench
    Insert
    dCas9-SpG_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216014-plasmid-map-sequence-id-431500

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  11. AA035

    Plasmid
    #216015
    Purpose
    Fragmid fragment: (Cas protein) deactivated Cas
    Depositor
    John Doench
    Insert
    dCas9-SpRY_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216015-plasmid-map-sequence-id-431509

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  12. pX2-Cas9

    Plasmid
    #85811
    Purpose
    Arabinose inducible Cas9 in a broad host range backbone.
    Depositor
    Ryan Gill
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Availability
    Academic Institutions and Nonprofits only
    85811-plasmid-map-sequence-id-177180

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  13. pLKO5d.SFFV.Cas9.P2A.BSD

    Plasmid
    #181981
    Purpose
    Lentiviral Cas9 expression
    Depositor
    Jan-Henning Klusmann
    Insert
    Cas9 (cas9 Streptococcus pyogenes (strain: NGAS638))
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    181981-plasmid-map-sequence-id-366611

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  14. AA007

    Plasmid
    #216002
    Purpose
    Fragmid fragment: (Cas protein) canonical Cas9; NGG PAM
    Depositor
    John Doench
    Insert
    Cas9_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216002-plasmid-map-sequence-id-431498

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  15. AA027

    Plasmid
    #216007
    Purpose
    Fragmid fragment: (Cas protein) nearly PAM-less
    Depositor
    John Doench
    Insert
    Cas9-SpRY_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216007-plasmid-map-sequence-id-431443

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  16. pRDA_734

    Plasmid
    #216097
    Purpose
    All-in-one, knockout, delivers Cas9 & gRNA
    Depositor
    John Doench
    Insert
    Cas9 [Sp]
    Use
    CRISPR and Lentiviral; Assembled vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216097-plasmid-map-sequence-id-431413

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  17. pMV_AA088

    Plasmid
    #216104
    Purpose
    Knockout, EF1a-driven Cas9 (Cas only)
    Depositor
    John Doench
    Insert
    Cas9 [Sp]
    Use
    CRISPR and Lentiviral; Assembled vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216104-plasmid-map-sequence-id-431403

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  18. AA017

    Plasmid
    #215928
    Purpose
    Fragmid fragment: (guide cassette) 2xPP7 & 2xMS2 in trRNA
    Depositor
    John Doench
    Insert
    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v14 [Sp]; CS2
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215928-plasmid-map-sequence-id-431437

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  19. AA022

    Plasmid
    #215930
    Purpose
    Fragmid fragment: (guide cassette) 2xPP7 & 2xMS2 in trRNA
    Depositor
    John Doench
    Insert
    U6_v1; sgCD4_v3 [Sp]; trRNA_v14 [Sp]; CS2
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215930-plasmid-map-sequence-id-431446

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  20. AA285

    Plasmid
    #215947
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Insert
    U6_v1; sgCD81_v1; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215947-plasmid-map-sequence-id-431462

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Showing: 41 - 60 of 839 results