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pSC101_TIMER

Plasmid

#103057

Purpose

low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

Depositor

Dirk Bumann

Article

Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

Insert

TIMER-bac

Use

Tags

Expression

Bacterial

Mutation

mutations: S4T and S179T

Promoter

constitutive ybaJp promoter

Availability

Academic Institutions and Nonprofits only

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pWJB30

Plasmid

#126505

Purpose

Salmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30

Depositor

Ute Romling

Article

Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

Insert

adrA-8xHis

Use

Tags

8xHis

Expression

Bacterial

Mutation

Promoter

PBAD30

Availability

Academic Institutions and Nonprofits only

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pRB133.2

Plasmid

#181950

Purpose

aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)

Use

Tags

mNeonGreen

Expression

Bacterial

Mutation

Promoter

PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106

Availability

Academic Institutions and Nonprofits only

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pFPV25

Plasmid

#20667

Depositor

Raphael Valdivia

Article

Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

Type

Empty backbone

Use

Tags

GFP mut3

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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SopB-EGFP

Plasmid

#183655

Purpose

C-terminally tagged Salmonella Typhimurium SopB for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB

Plasmid

#183657

Purpose

N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-SopB

Plasmid

#183670

Purpose

Inducible Salmonella Typhimurium SopB for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only

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pSKAP_AN

Plasmid

#122716

Purpose

pSKAP plasmid containing the unique DNA barcode "AN" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AN

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSKAP_AS

Plasmid

#122721

Purpose

pSKAP plasmid containing the unique DNA barcode "AS" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSKAP_AT

Plasmid

#122722

Purpose

pSKAP plasmid containing the unique DNA barcode "AT" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSKAP

Plasmid

#122702

Purpose

Designed for the incorporation of DNA barcode sequences for subsequent allelic exchange on bacterial chromosomes.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKAP_AA

Plasmid

#122703

Purpose

pSKAP plasmid containing the unique DNA barcode "AA" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSKAP_AB

Plasmid

#122704

Purpose

pSKAP plasmid containing the unique DNA barcode "AB" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSKAP_AC

Plasmid

#122705

Purpose

pSKAP plasmid containing the unique DNA barcode "AC" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSKAP_AD

Plasmid

#122706

Purpose

pSKAP plasmid containing the unique DNA barcode "AD" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKAP_AE

Plasmid

#122707

Purpose

pSKAP plasmid containing the unique DNA barcode "AE" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSKAP_AF

Plasmid

#122708

Purpose

pSKAP plasmid containing the unique DNA barcode "AF" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AF

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSKAP_AG

Plasmid

#122709

Purpose

pSKAP plasmid containing the unique DNA barcode "AG" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AG

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSKAP_AH

Plasmid

#122710

Purpose

pSKAP plasmid containing the unique DNA barcode "AH" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AH

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSKAP_AI

Plasmid

#122711

Purpose

pSKAP plasmid containing the unique DNA barcode "AI" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge