pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP
Plasmid
#171090
Purpose
CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
Depositor
Inserts
Sth1 dCas9
Chloramphenicol acetyltransferase
TagBFP
Chloramphenicol acetyltransferase
TagBFP
Use
CRISPR; Toxoplasma gondii expression
Tags
3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
Expression
Mutation
D9A, H599A
Promoter
TUB1 (Toxoplasma gondii), transcriptionally linke…
Availability
Academic Institutions and Nonprofits only