Multiplex Genome Editing with CRISPR-Cpf1
Type
Blog Post
...Cpf1 multiplexing: Transfection, lentivirus, and AAV
Cpf1’s ability to process its own pre-crRNA arrays...platforms: transfection, lentiviral transduction, and AAV transduction. In most of Zetsche et al’s experiments...array that can be processed by Cpf1.
Lastly, an AAV vector was used to multiplex edit 3-4 genes in a ...experiments, cells were infected with a 1:1 ratio of two AAVs, one expressing Cpf1 and the other a crRNA array...Experiment
A Match Made in Heaven: CRISPR/Cas9 and AAV
Resources on Addgene.org
Browse Our CRISPR Multiplexing...