Polymerase Chain Reaction Overview and Applications
Type
Blog Post
...relies on heat resistant DNA polymerase from the thermophilic bacterium, Thermos aquaticus (Taq). Taq polymerase...extension is repeated 25-35 times to exponentially replicate the target DNA of interest. This entire reaction...have been developed for different scientific applications. These PCR methods all use the same basic PCR...the quantity of DNA after a PCR reaction as the amplicon doubles every reaction cycle. However, it is common...goal is to amplify a specific target of DNA. This amplicon can be used in molecular cloning to create a plasmid...quantified by qPCR. qPCR is used in a variety of applications including gene expression profiling, studying...concentration, unlike qPCR where results can vary across replicates. ddPCR can be used to quantify DNA sequences ...