...26094 pET28a-LIC EF442785 Hexahistidine tag with Thrombin cleavage, KanR 26101 pET28GST-LIC EF456739 GST-tag...cleavage, KanR 26098 pCW-LIC EF460848 No tag, double ptac promoter, AmpR 26099 pFBOH-LIC EF456740 Hexahistidine...AmpR 26108 pFB-LIC-Bse EF199842 Hexahistidine tag with TEV cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression... Bac-to-Bac Expression System, AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with...proteins of medical relevance, and place them in the public domain without restriction. Many SGC plasmids from...cart, then complete the checkout process online. Click here for additional ordering information....

...technology and it's applications.... Genome Engineering Videos CRISPR Technology and Applications CRISPR-Cas Technology...Technology and its Applications Related videos Genome Engineering: Basic Principles Genome Engineering... If you have trouble loading this video, please click here . Please note: the chimeric RNA work (10:25...

...Polyhedrin pFastBac LIC and pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect... expression, Ligation Independent Cloning (LIC) Bacteria Lac, T7, araBAD p15TV-L - His-tagged ... Arrowsmith Lab Plasmids - More LIC bacterial cloning vectors pTD plasmid... GFP for mammalian expression pcDNA3 GFP LIC cloning vector - C-terminal GFP ...

...GST tag and TEV cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes the...containing a variety of tags can be found here . pBAD LIC Series (8-Series) Various Plasmids pBAD (arabinose...epitope tags useful for protein purification. pET LIC Series Various Plasmids T7-lacO (lactose/IPTG inducible...-PesaRlux controlling luciferase expression. pCW-LIC 26098 3x Tac promoter (hybrid of trp and lac promoters...Bacteria? E. coli are commonly used to store and replicate plasmids of all types, but beyond that, researchers...basic biology of a bacterial gene or redirecting metabolic pathways to produce a therapeutic compound, there...protein of interest. RK2 origin suitable for replication in many gram-negative hosts. pCri System Various...

...mCitrine 516 529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression pCS2+8CmCitrine...Zebrafish/Xenopus/C.elegans/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression...mOrange 548 562 49 6.5 2.5 hr Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression...mCherry 587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry...Zebrafish/Xenopus/C.elegans/Sea urchin pET mCherry LIC cloning vector (u-mCherry) - Bacterial Expression...

...Parkinson's Pablo Rivera-Fuentes 111723 pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111724 pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q73 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111725 pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q79 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111726 pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111727 pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111728 pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q73 HTT Flag CMV, P10...Huntington's Cheryl Arrowsmith 111729 pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q79 HTT Flag CMV, P10...

.... Feng Zhang LIC TAL Effector Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in...Golden Gate TALEN 2.0 48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b...

...bacteria for expression and purification ( pET28a-LIC or other pET vectors are recommended). Open Enzymes...

...(Lenti_sgRNA_EFS_GFP) 65656 Mammalian/Lentiviral LIC none S. pyogenes GFP Vakoc U6>sgRNA(F+E) 59986 Other...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...

...sensor, cytosolic or ER-targeted eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic and ...and small molecule indicator: implications for quantification of cytosolic Zn(2+). ACS Chem Biol. 2013 ...:7351. Tetsuya Kitaguchi cAMP (cyclic AMP) Fluorescent sensor of cyclic AMP (cAMPr) for mammalian or insect...sensor for cyclic AMP. Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. Justin Blau cAMP (cyclic AMP) ...:4003-4012.e6. Xavier Nicol cyclic di-GMP FRET-based biosensor for cyclic-di-GMP measurement Asymmetrical...Fluorescent biosensor of the cytosolic NADH/NAD+ redox state Imaging Cytosolic NADH-NAD(+) Redox State with...protein for NADH/NAD+ ratio Cytosolic NADH-NAD Redox Visualized in Brain Slices by Two-Photon Fluorescence...

...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods...of other antibodies. This has useful research applications, and is covered in the following Isotypes section...signaling molecules and are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments... molecules that can be distinguished in your application. The indirect detection method uses an unconjugated...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications...

...Selectable Marker PI Publication Bacteria Plasmid Gene/Insert Promoter PI Publication Drosophila Plasmid... Marker PI Publication Plant Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans ... Marker PI Publication Yeast Plasmid Gene/Insert Promoter Selectable Marker PI Publication Zebrafish Plasmid...Marker PI Publication Xenopus Plasmid Gene/Insert Promoter Selectable Marker PI Publication Parasites ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

... any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...each of the above serotypes and buffers. Please click on the plasmid name for ordering information. ID...When using the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite...When using the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite... using the AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye...using the AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite...Strategies for Overcoming Donor-Variation and Implications in Genome Editing. Sci Rep . Oct 19;6:35495....

...Resources Web References PAM Sequences Glossary Publications CRISPR Overview Class 2 C lustered R egularly... every genomic target. Due to its comparative simplicity and adaptability, CRISPR has rapidly become the...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR...-mediated DSB repair has important practical implications, because a population of cells expressing Cas9...requiring longer homology arms. Depending on the application, the repair template may be a single-stranded...and are thus well suited to directed evolution applications. Examples of these base editing systems include...be carried out by Cas9. Such multiplex CRISPR applications include: The use of dual nickases to generate...

... the original publication below. You can also download the full-text of the publication here . Article...bands from biallelic deletion clones with a proofreading polymerase and clone the amplicons ( e.g., with... of Visualized Experiments (JoVE). The video publication by Stuart Orkin's and Daniel Bauer's labs details...single-site small indels given the efficiency of biallelic modification, ease of rapid identification by ... sgRNA located within exons will enrich even monoallelic deletion clones for loss of function. This is...useful to characterize the non-deleted allele in monoallelic deletion clones. For small deletions (as the ...is critical that the deletion and non-deletion amplicons be easily resolved on an agarose gel for multiplexing...

...efficiency than NHEJ. Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome Editing...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...opposite strand) mutations. Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...repair (HDR). Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...RT template. Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a ...specific locus. Plasmid Gene/Insert Promoter PI Publication Interfere Catalytically dead dCas9, or dCas9 ...specific locus. Plasmid Gene/Insert Promoter PI Publication RNA Targeting Type VI CRISPR systems, including...

...headings. Click on the publication link to view all plasmids available from the article. Click on the PI...headings. Click on the publication link to view all other plasmids from that article. Click on the PI...Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle Vectors Search the table ...Description Vector Type Selectable Marker PI Publication Back to Top Do you have suggestions for other...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...library page. Find Pooled Libraries of Interest Click on different properties to create a custom filtered...Activation Human Zhang 3rd 10 96,458 Human CRISPR Metabolic Gene Knockout Library 110066 Knockout Human Sabatini...Knockout Human Olzmann 3rd ~10 13,920 Human lncRNA Splicing-targeting CRISPR Library 119977 Knockout Human...Knockout Mouse Yusa 3rd 5 90,230 Mouse CRISPR Metabolic Gene Knockout Library 160129 Knockout Mouse Birsoy...Knockout Mouse X.S. Liu 3rd 5 5 10 49,252 Human Metabolic Gene CRISPRa sgRNA Library 187080 Activation Human... Human Birsoy 3rd Varies 32,460 Mouse Metabolic Gene CRISPRa sgRNA Library 187081 Activation Mouse Birsoy...

...Selectable Marker PI Publication Bacteria Plasmid Gene/Insert Promoter PI Publication Drosophila Plasmid...Promoter PI Publication Plant Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans ...Promoter PI Publication Yeast Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...