...validated sensors in as little as one week (Pardee et al, 2016). Using toehold switch-based RNA sensors, our...riboregulators, called toehold switches, which allow for the rational design of RNA sensors that can detect ...the 48 sensors, targeting 24 regions of the genome, we have deposited the best performing sensors and constructs...Zika Sensor Constructs at Addgene As we report, we have embedded this next generation of toehold switch-based...put in place to provide community protection. Toehold switches for pathogen detection In 2014, as postdoctoral...exciting because it enabled gene circuit-based sensors to be used outside of the laboratory. The detection...patient samples (e.g.: blood, urine). Adapting toehold switches for Zika detection We and other members...

...isothermally, and detected through a toehold switch, an RNA sensor that can be used to detect the presence...the sequence of the toehold switch. For example, Green has also developed toehold switches to detect Zika...ribosome binding site, allowing for the gene on the toehold switch to be expressed. In this case, the gene ...

...benefit of this method. Find the toehold switch microbiota/biomarker sensors here Studying the bee microbiome...paper. At the heart of their method is the RNA toehold switch, a RNA hairpin that can be designed to bind...

...expression may be replaced by sensors that correspond to the desired inputs. Such sensors do not necessarily have...post-transcriptional recombinase activation via RNA toehold riboregulation could be a promising strategy for...PMID: 27463678. 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression...

...for a sensor for a different analyte, a sensor for gene-specific activity, or for popular biosensors in ...Reporters of miRNAs Toehold sensors for various target RNAs Explore the Fluorescent Biosensor Database designed...Calcium GCaMP-X genetic encoded calcium sensor Improved calcium sensor GCaMP-X overcomes the calcium channel...FRET-based zinc sensor for imaging in live cells (CALWY) Genetically encoded FRET sensors to monitor intracellular...+ FRET sensor, cytosolic or ER-targeted eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic...Genetically-Encoded Sensors. Anal Chem. 2017 Jan 3;89(1):711-719. Amy Palmer Zinc Cyan-Yellow Zinc FRET sensors ZapCY1...using optical sensors. Biochem J. 2010 Dec 1. Wolf Frommer ATP yAT1.03 FRET-based ATP sensor for yeast An...