Live and Let Dye: Self-Labeling Protein Tags
Type
Blog Post
Published
April 16, 2024, 1:15 p.m.
...Few
Complicated
Non-natural amino acids
1
Various
Many
Complicated
Applications
Organic...labeled through click chemistry. Although these multi-component systems can be complicated to implement,...having to clone a whole new construct for every application. Plus, the photophysical properties of chemical...advantages over fluorescent proteins, opening up new applications.
Figure 1: Live-cell labeling strategies...adapted from (Bottanelli et al., 2016) under CC-BY license.
Combining orthogonal self-labeling tags on...self-labeling tag and a fluorescent protein) enables applications like multi-color imaging (Figure 2). If you’...reused from (Ulrichs et al., 2023) under CC-BY license.
But these tags aren’t limited to fluorescent...