Which Fluorescence Microscopy Techniques is Best for Me?
Type
Blog Post
Published
Oct. 10, 2017, 1:57 p.m.
...red light instead of blue to excite a molecule of GFP. Because red light has approximately half the energy...light, two photons of red light are needed to excite GFP compared to only one of blue. Only at the focal point...infinitely small point of light – think of a single GFP molecule – will appear as a fuzzy 200-300 nm spot...