... Drosophila U6:1 and U6:3 Bullock 73914 pCFD5 multiplex gRNA plasmid Bullock 73915 pCFD6 tissue-specific... under control of the Drosophila U6:1 promoter Bullock 49409 pCFD2-dU6:2gRNA expression of gRNA under ...under control of the Drosophila U6:2 promoter Bullock 49410 pCFD3-dU6:3gRNA expression of gRNA under control...control of the Drosophila U6:3 promoter Bullock 49411 pCFD4-U6:1_U6:3tandemgRNAs expression of two gRNAs ...tissue-specific multiplex gRNA plasmid Bullock Cas9 plasmids ID Name Description PI 62209 pAct-Cas9 Expresses Cas9...genome engineering in Drosophila melanogaster. Bullock 62208 pnos-Cas9-nos Expresses Cas9 under control...genome engineering in Drosophila melanogaster. Bullock 62211 pAct-Fok1:dCas9 Expresses Fok1:dCas9 under...

...transcription Virmilion none, need Cas9 plasmid Bullock and Port pCFD2-dU6:2gRNA dU6:2 BbsI Injection or...transcription Virmilion none, need Cas9 plasmid Bullock and Port pCFD3-dU6:3gRNA dU6:3 BbsI Injection or...transcription Virmilion none, need Cas9 plasmid Bullock and Port pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6...transcription Virmilion none, need Cas9 plasmid Bullock and Port pAc-sgRNA-Cas9 dU6 BspQI Transfection ...

...Virmilion Bullock and Port pCFD2-dU6:2gRNA 49409 Fly BbsI none S. pyogenes Virmilion Bullock and Port ...49410 Drosophila BbsI none S. pyogenes Virmilion Bullock and Port pAc-sgRNA-Cas9 49330 Drosophila BspQI ...49411 Drosophila BbsI none S. pyogenes Virmilion Bullock and Port pU6-BbsI-chiRNA 45946 Drosophila BbsI ...gRNAs from Drosophila U6:1 and U6:3 promoters. Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing...functional gRNAs from a single precursor transcript Bullock pCRISPathBrick Bacteria Allows rapid modular assembly...