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Addgene
Showing: 1 - 20 of 37 results
  1. pnos-Fok1:dCas9-nos

    Plasmid
    #62210
    Purpose
    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Insert
    Fok1-dCas9
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    nanos
    Availability
    Academic Institutions and Nonprofits only
  2. pAct-Fok1:dCas9

    Plasmid
    #62211
    Purpose
    Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Insert
    Fok1-dCas9
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    act5C
    Availability
    Academic Institutions and Nonprofits only
  3. pCFD3.1-w-dU6:3gRNA

    Plasmid
    #123366
    Purpose
    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).
    Depositor
    Insert
    U6:3-gRNA
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. GFP-BICD2N

    Plasmid
    #64070
    Purpose
    N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)
    Depositor
    Insert
    BICD2N (1-400) (Bicd2 Mouse)
    Use
    Tags
    GFP and His-ZZ-TCS
    Expression
    Insect
    Mutation
    Codon optimized for Sf9 expression
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pDyn1

    Plasmid
    #64067
    Purpose
    Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).
    Depositor
    Insert
    DYNC1H1 (DYNC1H1 Human)
    Use
    Cre/Lox
    Tags
    His-ZZ-TCS-SNAPf
    Expression
    Insect
    Mutation
    Codon optimized for Sf9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 37 results