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Voigt-Lab-Repressors-icon.png Voigt Lab Repressor Plasmids


Deposited by Christopher Voigt's lab, these response function plasmids (pRF-) contain a transcriptional repressor, which controls the expression of a YFP output. Repressors are under the control of the IPTG-inducible Ptac promoter. Expression of the YFP gene is controlled by a synthetic constitutive promoter that contains a repressor operator sequence. The YFP output is repressed in the presence of IPTG. The various circuit plasmids achieve either the NAND (pLux-LmrA and pNAND-PhlF) or AND (pAND) behavior. To generate the NAND behavior, the following input concentrations were used: no inducer (-/-), 1 mM IPTG (+/-), 20 µM 3OC6HSL (-/+), and 1 mM IPTG and 20 µM 3OC6HSL (+/+).To generate the AND behavior, the following inducer concentrations were used: no inducer (-/-), 1 mM IPTG (+/-), 100 ng/mL aTc (-/+), and 1 mM and 100 ng/mL aTc (+/+).

Voigt-Lab-Repressors-for-Orthogonal-Logic-Gates.png

The work and corresponding plasmids are described in:

Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates. Stanton BC, Nielsen AAK, Tamsir A, Clancy K, Peterson T, and Voigt CA. Nature Chemical Biology. 2013. Dec 8. doi: 10.1038/nchembio.1411. PubMed.

Resources

Individual plasmids can be ordered via the links below:

ID Plasmid
49355 pRF-AmtR Add to Cart
49357 pRF-BetI Add to Cart
49358 pRF-BM3R1 Add to Cart
49359 pRF-ButR Add to Cart
49360 pRF-HapR Add to Cart
49361 pRF-HlyIIR Add to Cart
49362 pRF-IcaR Add to Cart
49363 pRF-LitR Add to Cart
49364 pRF-LmrA Add to Cart
49365 pRF-McbR Add to Cart
49367 pRF-PhlF Add to Cart
49368 pRF-PsrA Add to Cart
49370 pRF-ScbR Add to Cart
49371 pRF-SmcR Add to Cart
49372 pRF-SrpR Add to Cart
49373 pRF-TarA Add to Cart
49375 pLux-LmrA Add to Cart
49376 pNAND-PhlF Add to Cart
49377 pAND Add to Cart