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pAS2-1-Dhr1
(Plasmid #100002)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100002 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dhr1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    ECM16 (a.k.a. DHR1)
  • Tag / Fusion Protein
    • Y2H binding domain (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • Terms and Licenses

Depositor Comments

Addgene NGS results found S1073N, and an extra 30 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS2-1-Dhr1 was a gift from Susan Baserga (Addgene plasmid # 100002 ; http://n2t.net/addgene:100002 ; RRID:Addgene_100002)
  • For your References section:

    The SSU processome interactome in Saccharomyces cerevisiae reveals potential new protein subcomplexes. Vincent NG, Charette JM, Baserga SJ. RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117. 10.1261/rna.062927.117 PubMed 29054886