pFX-Pickles 2.34NES
(Plasmid
#100028)
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PurposeImproved FRET biosensor for the measurement of BCR-ABL activity.
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Depositing Lab
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Depositing OrganizationHokkaido University
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 100028 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 100028-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 100028-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepFX
- Total vector size (bp) 6128
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Vector typeMammalian Expression ; pFX vector is derived from pCMV vector.
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha and JM109 are suitable growth strains.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePickles-2.34NES
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)2101
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 100028-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 100028-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFX-Pickles 2.34NES was a gift from Yusuke Ohba (Addgene plasmid # 100028 ; http://n2t.net/addgene:100028 ; RRID:Addgene_100028) -
For your References section:
Improved FRET Biosensor for the Measurement of BCR-ABL Activity in Chronic Myeloid Leukemia Cells. Horiguchi M, Fujioka M, Kondo T, Fujioka Y, Li X, Horiuchi K, O Satoh A, Nepal P, Nishide S, Nanbo A, Teshima T, Ohba Y. Cell Struct Funct. 2017 Feb 2;42(1):15-26. doi: 10.1247/csf.16019. Epub 2016 Dec 8. 10.1247/csf.16019 PubMed 27928132
Map uploaded by the depositor.
