Purposeexpresses a chimeric OCT4-repressor fusion
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||100104||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerMichel Sadelain (Addgene plasmid # 22240)
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSN17 was a gift from Mark Ptashne (Addgene plasmid # 100104 ; http://n2t.net/addgene:100104 ; RRID:Addgene_100104)
For your References section:OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071. 10.1016/j.celrep.2017.07.071 PubMed 28813671