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p7142 pHAGE-P-CMVt-N-HA-GAW-ZFYV9
(Plasmid #100162)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100162 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PHAGE-P-CMVt-N-HA-GAW
  • Backbone size w/o insert (bp) 7800
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ZFYV9
  • Species
    H. sapiens (human)
  • Entrez Gene
    ZFYVE9 (a.k.a. MADHIP, NSP, PPP1R173, SARA, SMADIP)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p7142 pHAGE-P-CMVt-N-HA-GAW-ZFYV9 was a gift from Peter Howley (Addgene plasmid # 100162 ; http://n2t.net/addgene:100162 ; RRID:Addgene_100162)
  • For your References section:

    Proteomic analysis and identification of cellular interactors of the giant ubiquitin ligase HERC2. Galligan JT, Martinez-Noel G, Arndt V, Hayes S, Chittenden TW, Harper JW, Howley PM. J Proteome Res. 2015 Feb 6;14(2):953-66. doi: 10.1021/pr501005v. Epub 2014 Dec 15. 10.1021/pr501005v PubMed 25476789