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Addgene

YPRCΔ15Ω2
(Plasmid #101709)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101709 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDGB2Ω1
  • Backbone manufacturer
    GoldenBraid 2.0
  • Modifications to backbone
    flanking of the GoldenBraid cloning cassette with YPRCΔ15 arms of homology for integration in the yeast genome
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YPRCΔ15Ω2 was a gift from Elena Caro Bernat (Addgene plasmid # 101709 ; http://n2t.net/addgene:101709 ; RRID:Addgene_101709)
  • For your References section:

    Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria. Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, Navarro-Rodriguez M, Rubio LM, Caro E. BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 10.1186/s12896-017-0393-y. 10.1186/s12896-017-0393-y PubMed 29132331