Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pCMV-BE-VQRCas9
(Plasmid #101740)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101740 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    CMV
  • Backbone manufacturer
    origene
  • Backbone size w/o insert (bp) 3405
  • Total vector size (bp) 8532
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
  • Species
    R. norvegicus (rat), Synthetic
  • Insert Size (bp)
    5127
  • Mutation
    D10A, VQR mutations
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-BE-VQRCas9 was a gift from Shuo Lin (Addgene plasmid # 101740 ; http://n2t.net/addgene:101740 ; RRID:Addgene_101740)
  • For your References section:

    Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. Zhang Y, Qin W, Lu X, Xu J, Huang H, Bai H, Li S, Lin S. Nat Commun. 2017 Jul 25;8(1):118. doi: 10.1038/s41467-017-00175-6. 10.1038/s41467-017-00175-6 [pii] PubMed 28740134