pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532)
(Plasmid #102277)

• Purpose: For expression of His-GST-tagged human YTHDC1 YTH domain using mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)
• Depositing Lab: Samie Jaffrey
• Publication: Kan et al Nat Commun. 2017 Jul 4;8:15737. doi: 10.1038/ncomms15737.

Backbone

• Vector backbone: pT7CFE1-Nhis-GST-CHA
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size w/o insert (bp): 4323
• Total vector size (bp): 4977
• Modifications to backbone: None
• Vector type: For mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: YTH domain of human YTHDC1 (317-532)
• Alt name: YTHDC1
• Alt name: KIAA1966
• Alt name: YT521
• Species: H. sapiens (human)
• Insert Size (bp): 654
• GenBank ID: NM_001031732.3
• Entrez Gene: YTHDC1 (a.k.a. YT521, YT521-B)
• Promoter: T7
• Tags / Fusion Proteins:
  • HIS (N terminal on backbone)
  • GST (N terminal on backbone)
  • HA (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: ggcaagccacgtttggtg
• 3′ sequencing primer: gctagttattgctcagcgg

Resource Information

• Supplemental Documents:
  • pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532), no stop codon.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry