HyGCaMP6s
(Plasmid
#102558)
-
PurposeExpression of GCaMP6s in Hydra
-
Depositing Lab
-
Depositing OrganizationColumbia University
Located in United States of America -
Citations
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102558 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 102558-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 102558-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backboneDerived from pBluescript II SK+
-
Backbone manufacturerStratagene
-
Vector typeHydra Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameGCaMP6s
-
SpeciesHydra vulgaris
-
Insert Size (bp)1379
-
Tag
/ Fusion Protein
- Actin/GCaMP6s fusion protein (C terminal on backbone)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 102558-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 102558-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
HyGCaMP6s was a gift from Rafael Yuste (Addgene plasmid # 102558 ; http://n2t.net/addgene:102558 ; RRID:Addgene_102558) -
For your References section:
Non-overlapping Neural Networks in Hydra vulgaris. Dupre C, Yuste R. Curr Biol. 2017 Apr 24;27(8):1085-1097. doi: 10.1016/j.cub.2017.02.049. Epub 2017 Mar 30. 10.1016/j.cub.2017.02.049 PubMed 28366745
Map uploaded by the depositor.
