Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pRST11B-AS3_4
(Plasmid #102789)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102789 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

* Login to view industry pricing.

Backbone

  • Vector backbone
    pRST11B
  • Total vector size (bp) 6434
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    tryptophanyl tRNA synthetase
  • Alt name
    ScWRS
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1299
  • Entrez Gene
    WRS1 (a.k.a. YOL097C, HRE342)

Gene/Insert 2

  • Gene/Insert name
    suppressor tRNA
  • Insert Size (bp)
    100

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRST11B-AS3_4 was a gift from Andrew Ellington (Addgene plasmid # 102789 ; http://n2t.net/addgene:102789 ; RRID:Addgene_102789)
  • For your References section:

    Compartmentalized partnered replication for the directed evolution of genetic parts and circuits. Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD. Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. Epub 2017 Nov 9. 10.1038/nprot.2017.119 PubMed 29120463