TAL-L1-Tf1-Ctrl
(Plasmid
#103036)
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PurposePlasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 103036 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRN3p
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Vector typeT3 in vitro transcription for expression in mammalian cells
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTAL-L1-Tf1
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Tags
/ Fusion Proteins
- HA (N terminal on insert)
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site BsrGI (not destroyed)
- 5′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TAL-L1-Tf1-Ctrl was a gift from Maria-Elena Torres-Padilla (Addgene plasmid # 103036 ; http://n2t.net/addgene:103036 ; RRID:Addgene_103036) -
For your References section:
LINE-1 activation after fertilization regulates global chromatin accessibility in the early mouse embryo. Jachowicz JW, Bing X, Pontabry J, Boskovic A, Rando OJ, Torres-Padilla ME. Nat Genet. 2017 Aug 28. doi: 10.1038/ng.3945. 10.1038/ng.3945 PubMed 28846101
