PurposePlasmid encoding TagRFP-T
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||103998||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3224
- Total vector size (bp) 3920
Modifications to backboneSee "Notes On Plasmids In This Collection"
Vector typeBacterial Expression ; Low copy
Growth in Bacteria
Copy numberLow Copy
Insert Size (bp)696
MutationMutations relative to eqFP578 (R32G, K42R, K67R, L79F, I93V, N112D, I115L, N122R, S131P, R155E, H157R, S158T, Q159D, Y169H, H171I, S173N, F192V, H193Y, F194Y, M216V, K220R, R231K)
- Promoter proC
- Cloning method Gibson Cloning
- 5′ sequencing primer GCGTATCACGAGGCCCTTTC
- 3′ sequencing primer AAAGGGAAAACTGTCCATATGCAC (Common Sequencing Primers)
This FP derives from eqFP578. Please see "Notes On Plasmids In This Collection" document for further details and references.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEB2-TagRFP-T was a gift from Philippe Cluzel (Addgene plasmid # 103998 ; http://n2t.net/addgene:103998 ; RRID:Addgene_103998)
For your References section:Systematic characterization of maturation time of fluorescent proteins in living cells. Balleza E, Kim JM, Cluzel P. Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 2017 Nov 20. 10.1038/nmeth.4509 PubMed 29320486