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(Plasmid #104129)


Item Catalog # Description Quantity Price (USD)
Plasmid 104129 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    NEDD8-specific protease 1 (NEDP1)
  • Alt name
  • Species
    Brachypodium distachyon
  • Tags / Fusion Proteins
    • 14x Histidine tag (N terminal on backbone)
    • Maltose binding protein (N terminal on backbone)
    • Small ubiquitin-like modifier (SUMO) cleavage site from Brachypodium distachyon (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Supporting reference: A new set of highly efficient, tag-cleaving proteases for purifying recombinant proteins. J Chromatogr A. 2014 Apr 11;1337:95-105. doi: 10.1016/j.chroma.2014.02.029

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDG02583 was a gift from Dirk Görlich (Addgene plasmid # 104129 ; ; RRID:Addgene_104129)
  • For your References section:

    A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. Pleiner T, Bates M, Görlich D. J Cell Biol Dec 2017, jcb.201709115 10.1083/jcb.201709115