Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pBHt2
(Plasmid #104175)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104175 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAMBIA1300
  • Backbone manufacturer
    CAMBIA, Canberra, Australia
  • Backbone size (bp) 8960
  • Modifications to backbone
    pCAMBIA1300 was digested with with XhoI and BstXIA to remove the hph gene under control of the Cauliflower mosaic virus 35S (CaMV35S). The ends were blunted and ligated to a 1.4-kb HpaI fragment of pCB1004 containing the hph gene under the Aspergillus nidulans trpC promoter. The 3′ end of the hph gene was then ligated to the CaMV35S poly (A) termination signal.
  • Vector type
    Fungal expression
  • Promoter none
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pCB1004: Carroll, A. M., Sweigard, J. A., and Valent, B. 1994. Improved vectors for selecting resistance to hygromycin. Fungal Genet. Newsl. 41:22.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBHt2 was a gift from Seogchan Kang (Addgene plasmid # 104175 ; http://n2t.net/addgene:104175 ; RRID:Addgene_104175)
  • For your References section:

    Agrobacterium-Mediated Transformation of Fusarium oxysporum: An Efficient Tool for Insertional Mutagenesis and Gene Transfer. Mullins ED, Chen X, Romaine P, Raina R, Geiser DM, Kang S. Phytopathology. 2001 Feb;91(2):173-80. doi: 10.1094/PHYTO.2001.91.2.173. 10.1094/PHYTO.2001.91.2.173 PubMed 18944391