pEMS2144
(Plasmid
#104357)
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PurposeAAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104357 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneAAV2
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)SURE
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namessAAV-smCBA-EmGFP
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Alt nameEmGFP
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SpeciesSynthetic
- Promoter smCBA
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMS2144 was a gift from Elizabeth Simpson (Addgene plasmid # 104357 ; http://n2t.net/addgene:104357 ; RRID:Addgene_104357) -
For your References section:
rAAV-compatible MiniPromoters for restricted expression in the brain and eye. de Leeuw CN, Korecki AJ, Berry GE, Hickmott JW, Lam SL, Lengyell TC, Bonaguro RJ, Borretta LJ, Chopra V, Chou AY, D'Souza CA, Kaspieva O, Laprise S, McInerny SC, Portales-Casamar E, Swanson-Newman MI, Wong K, Yang GS, Zhou M, Jones SJ, Holt RA, Asokan A, Goldowitz D, Wasserman WW, Simpson EM. Mol Brain. 2016 May 10;9(1):52. doi: 10.1186/s13041-016-0232-4. 10.1186/s13041-016-0232-4 PubMed 27164903
Map uploaded by the depositor.
