PB-eDIO-ChR2-EYFP
(Plasmid
#104387)
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PurposePiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.
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Depositing Lab
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Depositing OrganizationTechnische Universitaet Dresden
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104387 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 104387-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 104387-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonePiggyBac
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Backbone manufacturerThe piggyBac vector backbone was a gift from George Church, Addgene plasmid #63800
- Backbone size w/o insert (bp) 8860
- Total vector size (bp) 10522
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Vector typeMammalian Expression, Cre/Lox ; PiggyBac
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChannelrhodopsin 2
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Alt namehChR2
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SpeciesChlamydomonas reinhardtii
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Insert Size (bp)1662
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MutationE134R
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Tag
/ Fusion Protein
- EYFP (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byKarl Deisseroth, Addgene Plasmid #35507
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 104387-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 104387-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-eDIO-ChR2-EYFP was a gift from Volker Busskamp (Addgene plasmid # 104387 ; http://n2t.net/addgene:104387 ; RRID:Addgene_104387) -
For your References section:
On-demand optogenetic activation of human stem-cell-derived neurons. Klapper SD, Sauter EJ, Swiersy A, Hyman MAE, Bamann C, Bamberg E, Busskamp V. Sci Rep. 2017 Oct 31;7(1):14450. doi: 10.1038/s41598-017-14827-6. 10.1038/s41598-017-14827-6 [pii] PubMed 29089561
Map uploaded by the depositor.
